FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_F03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_F03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614247
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8150.13268278070548167No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT7870.12812435388369825No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC7710.12551953855696488No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7310.1190075002401314No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT6580.10712303031191037No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA6220.10126219582676024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG259.4006915E-486.963433
TATATTC3500.053.8345073
CAATTCG2800.051.7344479
AGTAGAA5650.047.5198671
TATACGT657.320112E-444.6329962
GTAGAAA6400.044.2187581
AGCGAAA1401.402077E-841.465211
ATATTCA4450.040.6867034
CCACTAT2200.039.5804251
TTTAAAC5750.037.8101923
TGGTGCG1001.3996207E-436.2111667
CCTTATA2750.034.2862552
CGAAAGC1751.0118492E-733.128933
TAGAAAC9700.032.8727763
TTATACT3850.031.9786914
GAGCGAA1153.1443394E-431.5496141
ATATAGT3050.030.9289651
ACTATAT2859.094947E-1230.5134893
CATACTC1902.0820335E-730.5134893
ATAGTGG3100.030.390453
Produced by FastQC (version 0.11.5)