FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850525
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3150.052.9988021
CGAAAGC3950.044.0272453
GCGAAAG4650.037.432512
GATATAG2051.12522685E-831.8667281
GTATTAC7800.030.7091731
AGCGATA950.00475148130.4939658
GTATATA10700.027.8130661
GTATAGG2001.0555483E-525.4048611
TAACGAC4201.8189894E-1224.1538353
GATATAC1500.001498717524.1538353
ACGACTG4201.8189894E-1224.1424755
TATAGAC6700.023.814342
ATATACT7300.023.8131544
CCTATAC2451.9203708E-623.6608983
TATACTT7100.023.4624045
CTTAACG4353.6379788E-1223.360791
GTATTAT5350.023.0644931
TATTACA9450.023.0239662
AACGACT4750.022.873164
GTATACG2252.6269552E-522.5820981
Produced by FastQC (version 0.11.5)