FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT40.23515579071134626No Hit
GACATAATGGATCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCT40.23515579071134626No Hit
GGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGGCGTCCCAAGGC40.23515579071134626No Hit
CTCCCAGCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAA30.1763668430335097No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT30.1763668430335097No Hit
AAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTA30.1763668430335097No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG30.1763668430335097No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAGGCAATGAAAGAGTAT30.1763668430335097No Hit
AAACAAGGGTATTTTTCATTAATTGTCGTACTCCTCTGCATTGTCTCCGA30.1763668430335097No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG30.1763668430335097No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA30.1763668430335097No Hit
ATACTGACTCAGGGCCATTAATCTCCCACATCATTGATGAAGAGTAAGTT30.1763668430335097No Hit
CCATTGTTCCAACTCCTTTGACTGCAGCGCCAGCAGCTCCAGATCTCCTA30.1763668430335097No Hit
GTAGAAACAAGGGTATTTTTCATTAATTGTCGTACTCCTCTGCATTGTCT30.1763668430335097No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA30.1763668430335097No Hit
GTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAACTGACCCTCTT30.1763668430335097No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC30.1763668430335097No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC30.1763668430335097No Hit
AGTAGAAACAAGGAGTTTTTTTTCAACGGACTACTTGTCAATGGTGAATG30.1763668430335097No Hit
AAATAATAGAGGGAAGAGATCGCACAATGGCATGGACAGTTGTAAACAGT20.11757789535567313No Hit
GCATTATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTATTTAA20.11757789535567313No Hit
CTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC20.11757789535567313No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC20.11757789535567313No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG20.11757789535567313No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC20.11757789535567313No Hit
ACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGT20.11757789535567313No Hit
ATATAGTGGTATTACCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT20.11757789535567313No Hit
GCCTTAGCTGTAGTGCTGGCCAGAACCATTCTGTTCTCATGTCTTATTAA20.11757789535567313No Hit
CATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAA20.11757789535567313No Hit
CTATGGAGTAAAATGAATGATGCCGGGTCAGACCGAGTGATGATATCACC20.11757789535567313No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA20.11757789535567313No Hit
ATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAATAT20.11757789535567313No Hit
GATGCATACAGACTATTGAATACTGACTTAGCTAATAACGTCCTGCATAC20.11757789535567313No Hit
AGATTGGAGTGACAAGGAGGGAAGTTCACATATACTATCTGGAAAAGGCC20.11757789535567313No Hit
ATCTTCAAGTCTCTGTGCGATCTCGGCTTTGAGGGGGCCTGACGGGACGA20.11757789535567313No Hit
CAAGAGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTGATA20.11757789535567313No Hit
GAGCTACATCTTCTCCAATCTCATCGAGCTCTATCCAGATTGAATCGGTC20.11757789535567313No Hit
AAAATACAACGGTATAATAACTGAAACCATAAAAAGTTGGAGGAAGCGAA20.11757789535567313No Hit
CTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGA20.11757789535567313No Hit
ATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAGCA20.11757789535567313No Hit
TATTAGGCCAAATGCCGATTCGGTGGTCACAGCCCCCATCCTGTTGTATA20.11757789535567313No Hit
ACTTACCAGAGGACAAGAGCTCTTGTCCGCACCGGAATGGATCCCAGGAT20.11757789535567313No Hit
GAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGG20.11757789535567313No Hit
GTTAAACTGTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAA20.11757789535567313No Hit
CAACAAGAGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTG20.11757789535567313No Hit
ATGAAAGGACTTTGGATTTTCATGACTCAAATGTGAAGAATCTGTATGAG20.11757789535567313No Hit
ATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTGCT20.11757789535567313No Hit
ATTAATGGATTGGTTGTTGTCACCATTTGCCTATGAGATTTATGCTGGGA20.11757789535567313No Hit
AAACGTACGTTCTCTCTATCGTCCCGTCAGGCCCCCTCAAAGCCGAGATC20.11757789535567313No Hit
GGCATGGACAGTTGTAAACAGTATTTGCAACACCACAGGAGCTGAGAAAC20.11757789535567313No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGAGAATCTCTGTA20.11757789535567313No Hit
CTCTCTATCGTCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT20.11757789535567313No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC20.11757789535567313No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGACGATAAAT20.11757789535567313No Hit
ATTCTACACTGCAAAGACCCATTAGAACACATCCAGAAGCTGATTGCCCC20.11757789535567313No Hit
CTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGA20.11757789535567313No Hit
GAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACAAAGAAGAA20.11757789535567313No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA20.11757789535567313No Hit
GCTGAAAATCCTTCTAATTGTGGAGATGCATACAGACTATTGAATACTGA20.11757789535567313No Hit
GAAGTGGAGGAAACACTAATCAACAAAGGGCCTCTGCGGGCCAAATCAGC20.11757789535567313No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA20.11757789535567313No Hit
ACCCAAGGTCGATTCGAACCATGCCAATTGTCTCTGCACACACACATCAC20.11757789535567313No Hit
GTGTTGGAAAGCAAATAGTGGAGAGGATTCTGAAGGAAGAATCCGATGAG20.11757789535567313No Hit
GTATTGACCAATACTGACTCAGGGCCATTAATCTCCCACATCATTGATGA20.11757789535567313No Hit
ATCAAGTGCAAAATCCCAATGATACTTGCGGCAACAACAAGAGGATCACT20.11757789535567313No Hit
GGCCTCATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGG20.11757789535567313No Hit
CCTTCTAATTGTGGAGATGCATACAGACTATTGAATACTGACTTAGCTAA20.11757789535567313No Hit
AGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTC20.11757789535567313No Hit
GTACTGGGCCATAAGGACCAGAAGTGGAGGAAACACTAATCAACAAAGGG20.11757789535567313No Hit
CTATCGTCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA20.11757789535567313No Hit
GTAATGGTGTTTGGATAGGAAGGACTAAAAGTAACAGCTCCAGAAGAGGA20.11757789535567313No Hit
GAAGATGTCTTTGCTGGGAAGAACACCGATCTTGAGGCTCTCATGGAATG20.11757789535567313No Hit
CATTGGGACTCACCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTG20.11757789535567313No Hit
CTGCCTGGCCTGGCTAGCAACCTCCATGGCCTCTGCTGCCTGCTCACTCG20.11757789535567313No Hit
CCATTAATAAGACATGAGAACAGAATGGTTCTGGCCAGCACTACAGCTAA20.11757789535567313No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGTCAGGCCCC20.11757789535567313No Hit
ATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATC20.11757789535567313No Hit
CTTTGATGCAGGGTTCAACTCTCCCTAGGAGATCTGGAGCTGCTGGCGCT20.11757789535567313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG100.0064843297145.02
Produced by FastQC (version 0.11.5)