FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911819
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT25280.2772480064574219No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT20510.2249349925807644No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT17040.18687919422604704No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC16860.1849051182307015No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA16750.1836987384557681No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA16710.18326005490124686No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC16000.1754734218084949No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT15860.17393802936767055No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC15580.1708672444860219No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG14900.16340962405916087No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG14730.1615452189524456No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC14550.15957114295710004No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC14270.15650035807545137No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG13800.15134582630982685No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA13330.1461912945442023No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC12610.13829499056282002No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA12300.13489519301528044No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC12090.13259210435404395No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT11970.13127605369048023No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC11940.13094704102458932No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG11740.1287536232519831No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA10980.12041863571607962No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC10870.11921225594114622No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC10840.11888324327525529No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG10840.11888324327525529No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA10550.11570278750497633No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC10360.11361904062100045No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA9830.10780648352359404No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG9690.10627109108276972No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA9660.10594207841687878No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT9500.10418734419879384No Hit
CTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGA9360.10265195175796951No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG9290.10188425553755735No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGC100.0070856325145.03508145
ATCGCTA100.007111253144.86019
CCATAGG850.085.380391
TTAGTAG608.221832E-1084.668891
AGCGAAA451.2254077E-680.637031
TTCTGCG300.001933829472.51754145
TCGGTCC300.001933829472.51754145
GTAGAAA6800.066.16981
GGGTAGA1009.094947E-1265.3159941
GTACTGA4600.064.56436
GTCTAAC1550.060.8679541
TCCCAAC3250.060.2916871
TACTGAT5000.059.3958977
CCCAACA3500.055.951322
AACACTG3500.055.8807375
TGTTATG805.907914E-754.32855
AGGGTGT2300.053.5411266
GGCAGCC553.2150073E-452.74003145
AAGGGTG2350.052.4019555
CTGTGTC3850.050.79519
Produced by FastQC (version 0.11.5)