FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTTGCTAGACCGGACATGCAGCGGGTGCAGTAAATGATATACCGAGT14.3478260869565215No Hit
TAAATAATCAAACATTGTCCTTACTACATTATCACCCCTCCAAAAATTTC14.3478260869565215No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA14.3478260869565215No Hit
CTGCTGTTCCTCTCGATATTCTTCCCTCATAGCCTCTGGTACTCCTTCCG14.3478260869565215No Hit
ATATGATGAAATCAAATGCATGAAAACATTCTTTTGCTGGAAAGAGACTA14.3478260869565215No Hit
CAAAGATCCTGTTACCCAGACGCCGGAACACTCAAGTCTCTTTCCAGAGA14.3478260869565215No Hit
CTGCAGGACATTGAGAGTGAGGAGAAGATTCCAAGAACTAAAAAAATGAA14.3478260869565215No Hit
GTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGT14.3478260869565215No Hit
TCCATCTGATACTAATAGCCCTACCTTTGATTGGGTGTGATCCCACAGCT14.3478260869565215No Hit
AAACAAGGCATTTTTTCATGAAGGAAAAGTTAAATTCATTATTTTTGCCG14.3478260869565215No Hit
CCACGATACAGAAATTTTATCACTAGCAGTAGATTTAGGGATTGACAGAA14.3478260869565215No Hit
ATCTATCATAAAGAATATGCTTAAGTCTCTGTAGTGTCTTCACAGTATAA14.3478260869565215No Hit
GAGAGAGAGAGAAAAAGAGAGAGTGACCGCACAAGCAGGGGGAGTGGCAG14.3478260869565215No Hit
AAAGTGAAGTTCCAAGGCCATCATATTCCATGCTAAAGGCTCGACCATGC14.3478260869565215No Hit
GCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTG14.3478260869565215No Hit
TCGTCAGCATCCACCGCATTCTGCTGTTCCGCTCGGTATTCTTGGCTGAT14.3478260869565215No Hit
AAACCATACCTTCCATCTATCATACCACAGCGACCCCCATCAATCAAAAA14.3478260869565215No Hit
CTATAGCACACACTGTGGAGTTTGATTCATCTATTCAGATCAAGCTTTCC14.3478260869565215No Hit
CATCAAAGCAGCCACCTGTGTTGGAAAGCAAATAGTGGAGAGGATTCTGA14.3478260869565215No Hit
TCGCAGTCCCGAACTCGCGAGATACTCACTAAGACAACTGTGGACCATAT14.3478260869565215No Hit
CACAGGAACAATGCGCAGGCTCGCTGACCTAAGCCTCCCGCCGAACTTCT14.3478260869565215No Hit
CTAATAAACAAAGCGCCTCTGCGGGCCAAATCAGCATACAACCTACTTTT14.3478260869565215No Hit
GAGGAAACACTAATCAACAAAGGGCCTCTGCGGGCCAAATCAGCATACAA14.3478260869565215No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)