FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG11.7241379310344827No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT11.7241379310344827No Hit
TCCGAATTGGTTTGGTCGCTGTCTGGCTGTCAGTATGTATGCTTGAGTCC11.7241379310344827No Hit
TTTATATCATGTTCTCACTTGGAATGCAGGAACTTTTTTCTGACCCAAGG11.7241379310344827No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATGTTCAATC11.7241379310344827No Hit
CAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGA11.7241379310344827No Hit
GTATTCTCACAAGAAGATTGTATGATAAAGGCAGTTAGAGGTGATCTGAA11.7241379310344827No Hit
GCACACAGATTGGTGGAACAAGGATGGTAGACATTCTTAGACAAAATCCA11.7241379310344827No Hit
GAATAGGGAGGGTGTAGGCGGATTTGGCCCGCAGCGGCGCTTGGTTGCTT11.7241379310344827No Hit
ACTCTTCTTTTCCTAAATATACCAGAGCTAAATGGCATAAGCACGCCCTT11.7241379310344827No Hit
GCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCCAACTAATTCC11.7241379310344827No Hit
CTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAG11.7241379310344827No Hit
CACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAG11.7241379310344827No Hit
GGGCTAGGATTAAAACCAGACTATTCACCATAAGACAAGAAATGGCTAGC11.7241379310344827No Hit
TTACCAACCCTACGTCCCCTTGCCCAATTAGCACATTCGCCTTCTCTCCT11.7241379310344827No Hit
CGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCAT11.7241379310344827No Hit
TAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAG11.7241379310344827No Hit
GCCTTGTCGTGGAGGTCCGCCATACGGCCATGGGAGAGGAACAGGGTTCA11.7241379310344827No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATCGTTTGTTTTG11.7241379310344827No Hit
AGTTGGAACAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATCA11.7241379310344827No Hit
NNNNANNNGCAACTTCTTCCACGCCTTGGGGGGCCTCTCCAAGGGGGAAT11.7241379310344827No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.7241379310344827No Hit
ATAGGGTAGAGGCTTAGCTCATCTTTCAGTTTTGGAGTGTTCACTTTCAA11.7241379310344827No Hit
GGAGAAGTTCGGCGGGAGGCTTTGGTCAGCGAGCCTGCGCATTGTTCCTG11.7241379310344827No Hit
GTTAAAAAAACTATTACTTAATAGAAACCAAATCCACATATCCTAGCCAT11.7241379310344827No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG11.7241379310344827No Hit
GACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACAC11.7241379310344827No Hit
CGACACCACGCCCCCTCAGATACACACAGTTCCCACAATGCCATCAGCGG11.7241379310344827No Hit
AGAAGAAATAAGGCGAATCTGGCGCCAAGCCAACAATGGTGATGATGCAA11.7241379310344827No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG11.7241379310344827No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC11.7241379310344827No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11.7241379310344827No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCGTCCCGTCAGGC11.7241379310344827No Hit
AACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTA11.7241379310344827No Hit
CCTTAATTCAGGTTCGTCACTATCATATTGCTTCAAATCGCTTATATCTT11.7241379310344827No Hit
GAAATATTTATACCTCGAAAGTGCCTTTCAAGCTTGATACAGAAATTGAC11.7241379310344827No Hit
CATGAGAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCA11.7241379310344827No Hit
CTGTATTGGCCTCTAATGGCCTTAGGAACTAGAGACTGAAATGGCTCAAA11.7241379310344827No Hit
ATATGGAAAGAAGAAAAGCGCTACGGAATCGGAGGTCGCAGTCTCGCACT11.7241379310344827No Hit
ATAATAACCATAGGATCAATCTGTATGGCAATAGGAATAATTAGTCTAAT11.7241379310344827No Hit
CTGTTCAGACCTCGTTTAAGGAGACGATAAAGGCATTTGAAAAAAAGGCG11.7241379310344827No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA11.7241379310344827No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC11.7241379310344827No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT11.7241379310344827No Hit
ATACTGGAGATCCTCCATACAGCCATGGAGCAGGAACAGGATACACCATG11.7241379310344827No Hit
CCACAGCATTCTGCTGTTCCTCTCGATATTCTTCCCTCATAGACTCTGGT11.7241379310344827No Hit
CTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTG11.7241379310344827No Hit
GTCCAAAAAGAAATCAATAAGGCATCTGAATTGACTGATTCAAGCTGGAT11.7241379310344827No Hit
GCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAA11.7241379310344827No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG11.7241379310344827No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCATTGCCATCATCCCC11.7241379310344827No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG11.7241379310344827No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT11.7241379310344827No Hit
TGTTTGGCCAGTATGGATTGCATATAGTAGCATTGCCGGCACGCCGTCAG11.7241379310344827No Hit
GGTTCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT11.7241379310344827No Hit
CCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAGG11.7241379310344827No Hit
ATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACT11.7241379310344827No Hit
AGTAGAAACAAGGTACTTTTTTTGGACAGTATGGATAGCTGTCTCTTATA11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG50.0145.05
TTATACT50.0145.04
CTGGAGA50.0145.09
TATTCTC50.0145.0145
CTTATAC50.0145.03
TACTGGA50.0145.07
CCTTATA50.0145.02
ATACTGG50.0145.06
CCCTTAT50.0145.01
ACTGGAG50.0145.08
Produced by FastQC (version 0.11.5)