FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032428
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT30880.29910076053729656No Hit
GAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAACCTT29580.2865090834421383No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT21040.20379145083240674No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA19670.19052176035520152No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18570.17986726435160613No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG18380.17802694231462146No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT18140.17570232500474608No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA17990.174249439186074No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC17450.1690190502388544No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC16980.16446667467368184No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC16930.16398237940079113No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC16750.16223891641838464No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA16750.16223891641838464No Hit
GGCATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC16460.15943000383561856No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC16010.15507134637960227No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT15950.1544901920521334No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG15100.14625717241299152No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA14660.14199537401155335No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA14600.1414142196840845No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA14210.13763671655553705No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG14210.13763671655553705No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC14120.13676498506433377No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG13820.1338592134269896No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC13680.13250318666289562No Hit
CCTTTGGGTGACTCTCCAATGGGCCATGTTTCTGATCTATTCTCAAAAAA13610.13182517328084864No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA13470.13046914651675467No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA13300.12882254258892628No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA13200.1278539520431449No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT13180.12766023393398862No Hit
GGCAATGAAAGAGTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTG13170.1275633748794105No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT12590.12194554971387836No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC12360.11971779145858114No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA12180.11797432847617462No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA12020.11642458360292436No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCT11850.114777979675096No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC11780.114099966293049No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT11490.11129105371028294No Hit
ATCTCAATGCATGTGTTAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA11360.11003188600076713No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC10640.10305803407114104No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG151.2288251E-4145.03526145
AGCGAAA750.0116.0620041
GCTCACG256.457156E-6116.02821145
CTTCCCG404.807589E-9108.77645145
GGGTAGA1150.082.000331
AGGACGC406.655261E-572.51763145
CAGGGTG754.9076334E-967.5749365
GTACTGA3650.067.449236
GTAGTAT1350.064.478891
GTAGAAA6100.064.214631
CCGTATA350.003563100262.15797145
TCGGTCC350.003563100262.15797145
TACTGAT4000.061.5474247
CCATAGA606.784212E-660.448961
AGGGTGG851.3184035E-859.6307266
CAAGGGT3850.056.4196554
ATATAGA1800.056.3151784
GTCAATT651.0964952E-555.7449342
TATTAGT400.006038161554.404061
AGATGGG400.0060451254.388226145
Produced by FastQC (version 0.11.5)