FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACTATTATATCTTCAAATGCAGCAGAATTGCATGCCATCCACA12.7027027027027026No Hit
GGGACAGGGTACACAATGGATACATTCAACAGGACCCATCATTACTAAGA12.7027027027027026No Hit
GTTTTTTCTTCCCTATTGTCCTTTGCGTGACCCTCTTCTTGGTCATGTTG12.7027027027027026No Hit
AGATAGGAGATATGCTTCTAAGAAGTGCAATAGGCCAGGTGTCAAGGCCC12.7027027027027026No Hit
GCTGGAAACTCTAATATTACTAAGGCCTTTCACCGAAGAGGGAGCAATTG12.7027027027027026No Hit
ACATTCCACGGCTCACAGATCCACATCCATATAATAATACACTCCGGATT12.7027027027027026No Hit
GATGAGGCGTTGCCGCCTCCAATGACGGCAACAAATAGAGCGGATGATTG12.7027027027027026No Hit
ATATACATCCGATCACAATTGGAAAATGTCAAAAATATGTAAAAAGCACA12.7027027027027026No Hit
GTATACTTGGGATTGGGCCCGTGCTATGGGGGAGTCGTGATAGCCTGTGT12.7027027027027026No Hit
GGGCCATATCTCTTGTATGATGTAATTGGGTATCACATTGCCATCATCCA12.7027027027027026No Hit
GTGGGCTGGGCGCCACGTGCTGTGTCGGGTGGTGTTCACGGTCTCGGTGC12.7027027027027026No Hit
GTGTGTACATTGAAGTTTTGCATTTAACACAGGGAACATGCTGGGAACAG12.7027027027027026No Hit
GGTTGCTAGCCAGGCCAGGCAGATGGTGCAGGAAATGAGAGCCATTGGGA12.7027027027027026No Hit
ACTGAATAGAAAGCTTAAGAGGTAGATAACATTAAATGGGGACAAAGAAA12.7027027027027026No Hit
ACAGAGGACTCAGCTTCAATCATACTCTCTATTTGTTGAAGTGATTGGAG12.7027027027027026No Hit
AGCAGGTACTTAACCAAAAAGGAAGACATTGTGCCACAATGCTTAAATCA12.7027027027027026No Hit
GCAGCTGTACACTCGAGGAGGAGAAGTGAGAAATCATGATGTTGCCCTCA12.7027027027027026No Hit
ATTTTGACGAGAGCAGGAATAAATATCTGGAAGAACATCCCCCCCCGGGG12.7027027027027026No Hit
ACCGAAAGCATGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTT12.7027027027027026No Hit
ATCAAAAACATAAATTGCAAAATCCCAATGATAATTGCGGCAACAACAAG12.7027027027027026No Hit
TAGGAGATCTGCAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAATGG12.7027027027027026No Hit
CCTATCAGTGGATCATCAGAAACTGGGAGACTGTTAAAATTCAGTGGTCT12.7027027027027026No Hit
CTCAATATGCCTCTCAACAACTTGAGGGGTATTCCGCTGAATCTATAAAA12.7027027027027026No Hit
CTGTGAACAGATTTCTGAATCACAGAATAGATCTCATATGCAAATGGTGA12.7027027027027026No Hit
CCAGCCATTTGCTCCATAGCCTTAGCTGTAGTGCTGGCCAGAACCATTCT12.7027027027027026No Hit
CTTCCGTAGAAGGCCCTCTTTTCAGACCGAGTTTAAGGAGACGATAAATG12.7027027027027026No Hit
CTATACACCATTGCCACCACCATGCTAGAAATTCCAACCGGTCTCGAATA12.7027027027027026No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC12.7027027027027026No Hit
ATGAAGGACAAGTTAAATTAATAATTTATGACGTCTGAGATCTTCAAAGA12.7027027027027026No Hit
TGGTAATGCTGATTGGATAGGAAGGACTAAAAGTAACAGCTCCAGAAGAG12.7027027027027026No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT12.7027027027027026No Hit
TCTTTCGCCTTCCCTCTTAAGTAGTACATTTGCTTATCATGGGAATTATT12.7027027027027026No Hit
GCTCGAGGATGATGTAAAACTCAAAGTGGACAAGCATATGATTTTGTGAG12.7027027027027026No Hit
AAATAATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGA12.7027027027027026No Hit
GTTTTAGGTCTTCCTTCTTTGGTCCTACATTTGCTGATCATTGCGATCAG12.7027027027027026No Hit
CCTATCTTCTTTGCCCAGAATGAGGAATCCCCTTAGAACAGCGGATTCCA12.7027027027027026No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTCCTTCATATA12.7027027027027026No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)