FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_E01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_E01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828636
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG20420.24642907138960896No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA18860.22760295232164665No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC17700.21360404327111063No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG15240.18391670166393928No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC14160.17088323461688848No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT13790.16641806535076922No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA13470.16255629733682825No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC13460.1624356170863926No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC13460.1624356170863926No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC13320.1607460935802934No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA13090.1579704478202733No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC12980.15664296506548112No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT12790.15435004030720365No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA12450.15024691179239136No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC11590.13986841025492497No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG11200.1351618804879344No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC10880.13130011247399342No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT10370.12514541970177498No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC10330.12466269870003235No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT9760.11778392442519997No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG9610.11597372066866514No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAAAACGTTCTTA9340.11271535390690243No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA9170.11066378964949629No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG8790.10607794013294136No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA8720.10523317837989178No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT8710.10511249812945611No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA8590.10366433512422825No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT8540.10306093387204998No Hit
CTCTAATATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA8470.10221617211900037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA607.2759576E-1296.7226941
TAGGGGC301.5975751E-596.68762145
GTCAATT750.096.664252
TTAGTAG702.910383E-1182.905161
GTAGAAA6650.075.269161
GGGTAGA1450.075.0434651
TATTAGT300.001931179872.542021
CCATAGG702.7994247E-972.5420151
GTACTGA1650.070.22496
TACTGAT1900.064.7963267
AGGGTGT4300.052.209356
ACACATA701.6935559E-551.79694145
AATTATA1405.456968E-1251.7312855
TAGAAAC10150.049.998752
AAGGGTG4500.049.891955
CAAGGGT6850.049.6862458
GGTAGTT1900.049.5501337
TCCCAAC3700.049.0148771
CCCAACA3850.047.0767442
AGGTAGT2000.047.0726246
Produced by FastQC (version 0.11.5)