FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAAAGCTCTCCAGGCATGGGAGATGAATACAAGCAAGGAGGTGGGT13.125No Hit
TAACTATATTGTTCTTCCAATCCATTAGCATGGATAGTCTTCCCCTTAAT13.125No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT13.125No Hit
ATGCTAGAGTCCCGGTTGCGGGTCGGTTCCCACCCCACGGCTCCTTGCCC13.125No Hit
GGGAGATGAAGCATGAGAGACCGTGGACTCTGAGAAAAACTGAGGGTTTT13.125No Hit
TTTATAAGTTGTGAAAAATAGTTCTTCTAAAGTATAAAAATCTTTTTACA13.125No Hit
GCAACAGAGTTGGCTTGTTTTTTTTTTTGCACCACAACCCGAGCCACCAG13.125No Hit
ACCATAATTGATGTAAATAAGAATTATTTTGGTAGCTTTAGTTCTGTAAG13.125No Hit
GCCCTAATTAATCCATGATTAGCAGCCCTTTCTTGCTCAGCAAATACAAA13.125No Hit
GGGCCACACTGAGACAGCCCTTCTGTCGCATCAATTTTATGCTACAAATA13.125No Hit
CTTGAGGAGGTGGAACCACTGGCTGGGAGGGTGGGGGTGACTTGGGAGCA13.125No Hit
ATGTATTACTGACTTAGTGTGAACAGGTTTCCGAGTGCTTATTTCAAAAC13.125No Hit
GAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAA13.125No Hit
ATATAATCAAGTATGGTTTCATTTGGCCAAGGAAGAAAGGGTGTAGTAGT13.125No Hit
ATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTG13.125No Hit
ATGAAAAATCCTCACTTTCTATTTTACTGTGTAAGCATGAACCCATGTCT13.125No Hit
GCCCTGTGCTGCCAGGTGGGGGTTCCCAGGGCTGTGACTGGACAGCTTAG13.125No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC13.125No Hit
GCCTGAGAAATCGCAGAGAACACGAGCTGGTAGATGACGTTCTTGGAATT13.125No Hit
TTATAATATAGTTACTGGTACCTAAAGGAAGATTCTGTAATACTGAATTA13.125No Hit
GGGGAGGCGGTCCCCTGCTGTAGACTCTGGTTTCTGCTCTGAGTGCAGTG13.125No Hit
CTAGAAGTCTGGAACGTGATACCTCAAGATTTGCTTGCCTTTTTTAAGGT13.125No Hit
AGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACAC13.125No Hit
AACTGGACCAGAGCGGCGCACTAAGTTCACGTGATGCTGCAGCTTATCTG13.125No Hit
GGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGTCCCCCGGTG13.125No Hit
GAATTAGGCTGTGCTTGCCACCCATGCCCGCATGACAGCTGCTGGTCCTA13.125No Hit
GCATAGCGCCCACCCGGCAGGAATGAGCGGGCATCCACAGAGCTGAGCAG13.125No Hit
GCTATAAGAGAGTAATGAAGAGAAAAGAGAAAATAGATCCAGCACGAGCA13.125No Hit
GGGTCGCGTCGTGGTCTTTCAGTATTCCGATGGAAAACTACTGCCTGTGC13.125No Hit
CTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCT13.125No Hit
TTCTTAAGCTAGCATTGCTTCATCACCTCTAATCCTGAATTTTCCGCGCG13.125No Hit
CTGGTGGAGTGGAGAGCCCGGTGCTGCTGCCGGTGGTGCCGGCTACTCAG13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)