FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.17152658662092624No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT40.17152658662092624No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC30.1286449399656947No Hit
CATTATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA30.1286449399656947No Hit
GTACAAATTCTGCAGCAAGGCAGCCACTGTGCTCTTCCCGGACCCATTGG30.1286449399656947No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.1286449399656947No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30.1286449399656947No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.1286449399656947No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC30.1286449399656947No Hit
GGTCAAGGAACCAAAGGAGGGATAAGGGCCAAGGATCTGGGCAGTTGGAC30.1286449399656947No Hit
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC30.1286449399656947No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT30.1286449399656947No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC30.1286449399656947No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.1286449399656947No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.1286449399656947No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA30.1286449399656947No Hit
GATGTATTATGCCCCAATACTCAGCAGCAGGGAAATGAGGATCTTGGAAA30.1286449399656947No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.1286449399656947No Hit
TCCTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCAC30.1286449399656947No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCG100.00664057145.09
CCCCAGA100.00664057145.04
Produced by FastQC (version 0.11.5)