FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTGGAACAGCTTGCGATTAGTTGGTCCTTCATAGCTCAGTCCGCA15.555555555555555No Hit
CCTCTGTGAAGAATATGCTGGGATATACTTTTTAACCATTCAGGTTAGCC15.555555555555555No Hit
GTACACATATATTACCTATAGAAAGCCATGTGAGCACCAATGGAAACTCA15.555555555555555No Hit
CATCCGGAGCAGGGCAAATATAATGTTCTCAAAAAAAATGTAAAGACTAG15.555555555555555No Hit
TGCATTGACTAAAGATCCAGATCAAGGAACATATGTGATGGATTCTGCTG15.555555555555555No Hit
GAATGGGATACCTTCACAACCTCCAGAGCAGAAATGAAAAGTGGCTAGAG15.555555555555555No Hit
GGATAAACCATGGACCCAGTAAACAGAACCCCCCAATGCACCCCAGCGCC15.555555555555555No Hit
GCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTGTA15.555555555555555No Hit
CATCAAAGTCTACTTTTTCTGGTGCCTTATTTTCACCGAGTGCCCACTTC15.555555555555555No Hit
TATATAAATACTATGCAGACATCAAAAGAAATGAAATCTTGCCATTTGCG15.555555555555555No Hit
AACTACGTACCATTGAAAAATTAGCACATTAGCATTCTCTACTTTTGCCA15.555555555555555No Hit
AATATAAAACAGGATGTGAACAGTGCCAACATAAGCTGCTTTTTGTATAG15.555555555555555No Hit
CTTCAGTGGTGGGAGGACACATACCAACTCATTTTAGTTTTTCAAATGAC15.555555555555555No Hit
GTTTGTGCCAATTGTGAAAAGAGTCCTGATGCCCAGCATCGGGAGCACAT15.555555555555555No Hit
GTACAGCCTCGAAGTATAACCAGGCATCAAGAAGGTCCAGATACCCAATT15.555555555555555No Hit
GTCTTCTTGCCAGCTGGGAGGGAGGAGCAGTGGAGAGGCAAAGGTTGATT15.555555555555555No Hit
GGTCAGGTGCAGGCAGCTGAGGTTAGAGGCCAGGTTTCCCCCTGCTGCGG15.555555555555555No Hit
CCATTTGAAAGGATGACAATCCGACTCTACATTACCATAAAATTCCAGAG15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)