FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753966
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8880.11777719419708581No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC8270.10968664369480853No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA8170.10836032394033683No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA7570.10040240541350672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1700.055.475771
CAATTCG3000.041.0435339
AGTAGAA7250.038.023711
GTAGAAA7350.036.497552
CGAAAGC2451.8189894E-1235.4828643
TAGAAAC7750.034.5862543
TATACGT1301.6652526E-533.429124
AGGTCGT2807.2759576E-1231.0413286
GGATAGC4450.030.9250685
ACATACG950.004725960530.5271112
TGTATAC1952.6052658E-729.7443662
CCTTATA4650.029.6244282
GGTCGTT2451.9917934E-929.5612097
GCGAAAG2951.4551915E-1129.4922962
GATAGCA4700.029.2801176
GACAGTA3600.028.1652647
GAGTTAT2954.8748916E-1027.0059197
TTATACT6450.026.9506114
TCGTTTT2803.6379788E-1225.8677739
TGGTGCG2001.0736036E-525.3487368
Produced by FastQC (version 0.11.5)