FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGATGTGGACTTCCCTTTTCGCGGGCCATGGCGTCATCCTGTGCCT11.1764705882352942No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT11.1764705882352942No Hit
GAAGAATCCCCCCCAGGAATATTTGAGAATTCATGCCTTGAACCAATGGA11.1764705882352942No Hit
GTTAGGAGCAGTGACTGTGGTGAAGAGTGTGGTAACAATTACAGCAAAGA11.1764705882352942No Hit
GTCTTTTGGTGTGGCTGGCTTGCGGCTCGGCTGCAGTTGAAGTTTTCAGG11.1764705882352942No Hit
GTGCAACACTTCAATATAAACAATGCCTGTTCCGCCGGCTACTGGGAGAA11.1764705882352942No Hit
GCTTAATGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATA11.1764705882352942No Hit
TTCCTATATTAAATTCCCTGCTTGAAATATCATGTGGTTTCTGGTTTCCT11.1764705882352942No Hit
GTCTAAGTTCCTGCTGATGGATGCTCTGAAATTTAGTATTGAAGACCCGA11.1764705882352942No Hit
CTATATGTCCCTGATAGAATGATGTTAACAAGCTCCTGCAGTTTCTACTC11.1764705882352942No Hit
GGATACAACATTCCACTAGCATACAATAACCAAAAATTACATGAGTTAAA11.1764705882352942No Hit
TAATAAACCTTCTCCCCTTTGCTGCTGCCCCCCCAGAACAGAGTAGGATG11.1764705882352942No Hit
ACACAAGATTCCCCTCTGGCTTAGTTGCCTGCTCCTGGCTGCCAAGGTGC11.1764705882352942No Hit
TCATATCTCAGGGTCTGAGACTGAGCCCTGCATCAGACTCTGCGCTCAGT11.1764705882352942No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC11.1764705882352942No Hit
ATATATTTCTCCTGTGCTTACCTAAAAAAAATTTGCATTCAGTATAAAAT11.1764705882352942No Hit
CAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATAAGA11.1764705882352942No Hit
GAATATAGCATAGACTACCTATCGGGTGATAAGTCAATGAATGAGTAAAT11.1764705882352942No Hit
TGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAG11.1764705882352942No Hit
TGTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAAT11.1764705882352942No Hit
GGACGGCTCGCGGCGGAGGGCGGGGGGCTGGAATGAGCTGCGTTGGCGGG11.1764705882352942No Hit
TTATCACTCTGCGCCAGCGCGGCGCCCCATGGTGGTAGTATTTTGTATGC11.1764705882352942No Hit
CGTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGAT11.1764705882352942No Hit
AGATAGTGGTATGCCCTCCCCCTCGTGACTCGGGTCGTCAAGACTTAATT11.1764705882352942No Hit
NTAACGTCCTTGGCGGAATCCTGAACAATTTGTTTGATCAGAGAACAAGC11.1764705882352942No Hit
CATTGACTGTGAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGC11.1764705882352942No Hit
GTATCGCCTGTTGCCGTCCTTGGTGCATCTCGGTAATGTGTTTGCCCTGG11.1764705882352942No Hit
GTGTGGGGGGGGGGGGTGGGGGGGGGGGGTGGGTGTTGGCCGTTGGGGTG11.1764705882352942No Hit
TTCTTGATCCGTCAAGAATCGAAGTCGACCATGGCAGAAATCAGGGCCCT11.1764705882352942No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT11.1764705882352942No Hit
GGGCTGGAGGCCCTTCCTGGATGTGCCAAACTCCTACAGACTCCCTACGT11.1764705882352942No Hit
CGCCTGCGCTCCGGTTGCCAGAGGGATCAGTGCAGGGCGGCTGCATGCGG11.1764705882352942No Hit
GGCTAGGTCATAGTGTTGGCTCCATGTGCTGTAGGGCCCTGGGTTCTCAG11.1764705882352942No Hit
GTCTTGATGTTCACTGAGTCATATATAGAACTATCGAATCACTTTATTGT11.1764705882352942No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC11.1764705882352942No Hit
ATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCACTTCAC11.1764705882352942No Hit
GAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGC11.1764705882352942No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTGACTGACCCG11.1764705882352942No Hit
CTTCACCATAAGCTGCGTAACCTGTGAGAGTTGAGCTTTCTGAGCCTCAT11.1764705882352942No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT11.1764705882352942No Hit
TTATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC11.1764705882352942No Hit
GGGTGCGCCGTGGGCGGGCAGGTGGGGGTCGAATGGGGCGGCACCCGGGA11.1764705882352942No Hit
CACTTGATGAAGAGAGCAGGGCAAGAATAAAACCTAGGCTTTTACCTATA11.1764705882352942No Hit
ATATTACCCTTAGTTCGAAAAGGTCGAAAGGTGGACACATGGTCCGTTCG11.1764705882352942No Hit
GTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCA11.1764705882352942No Hit
ATCCTCAACCCTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGT11.1764705882352942No Hit
CTGCAGCTCTTCAGCCCGTAAGCAATGTTGTCCCTCACAGAACCCGAGAA11.1764705882352942No Hit
CACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATTA11.1764705882352942No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC11.1764705882352942No Hit
ATATGATGTCGTGTGTTCATAATCTTGAATTCATACTCAGATTCAGTAAA11.1764705882352942No Hit
GTGTCTCGTTGGGAGTAAGTATTCTAGTGGCCAGTTTTCGGTGAAGTCCC11.1764705882352942No Hit
TCCCTAGCCTTGGCCGTATTTATAGCCAGCAACATGATGTGTGACTGCCG11.1764705882352942No Hit
CCCAACGATTGTCAATTCATCATCCCCGTCATCTTGTCTTATTTGCAGTG11.1764705882352942No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC11.1764705882352942No Hit
GCTGCGGGGAGTGGGGGGCGGTGTGACGTGTGGGAATAGGGCCGCGGCGC11.1764705882352942No Hit
GAACTTGATACCTCCAGATTTGCTTTCCTTTTTTAAGGTTGTTTGGCTAT11.1764705882352942No Hit
GCATTTTGCAGAGAATAAGAGCAGTGAGAGATCGAATGTCGCAGTCGCGG11.1764705882352942No Hit
GACAAGAGTTGTAAAACCAGACATGCTCATGGACCAAGCAACGGCACTCT11.1764705882352942No Hit
GTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACC11.1764705882352942No Hit
GAAGGGGGAGAAGCTCGGATACTGCCCGCTGCCACTGCTCCTGCTCCTGC11.1764705882352942No Hit
ATCATACCTACGATGATAAATCTCTAGCTATTACTGCAAAGCCCGAGAGT11.1764705882352942No Hit
TCCCCACCCCGGTCAGCCCACGTGGTGCAAACTTCCAGCATGCGCATAGC11.1764705882352942No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGC11.1764705882352942No Hit
GCTTGGGACGCGAGCCCAAATCCCGCCACGTCATTAACCCAAGCAGCTGC11.1764705882352942No Hit
GAACTGAGGCTTTTCAAAATTAAATAAGGGCCAGTTTCCTGTTCATGGTG11.1764705882352942No Hit
CGATAACCTCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTT11.1764705882352942No Hit
GCCAGAAGGGGAGAAAATCGGGGACAAGTTTCTCTGCGGAGTTCAATCGC11.1764705882352942No Hit
GTCTCTACCAACGTGCTCCTGGACGGAATGAGGACAGAAAGGAAGGGCCA11.1764705882352942No Hit
GTCTAGAAGTACGTAGGCAGGGGAAGAGGGTAAAAGGGAATGGGCTGGAT11.1764705882352942No Hit
CCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCAT11.1764705882352942No Hit
GAGCAATTTGGGATTTCACGCCCTTTTGAAGCCGAGCGGCATCTACTGAA11.1764705882352942No Hit
GCTGGGGGGGTCGGGGCGGGGGGGGGGGCAGCAGGGGGGGGTGGGGGGGG11.1764705882352942No Hit
CCTTTGGGAAGTTTGGCGGAGAAAGGTATGATGGGGCAAAAGATGGTTGG11.1764705882352942No Hit
TACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACA11.1764705882352942No Hit
GGTTGGAAGGGTCCTTAAAAGACCTGCTGAAAGCCAATGGAACTCCACTA11.1764705882352942No Hit
GTGTTCAAGCCCTCCACTGTGTATAGAAAGTACTGAAAATTAAAGGCTTT11.1764705882352942No Hit
TTCCCAGACTGTCCGGCGGAGCCGTGCGGGGCTCCTCCCCGCACCCGCTG11.1764705882352942No Hit
CTTCCAGTTTTTTTTTTAAGCTGTTTGTTTTTGTTTTGGCGGCCGGCGAT11.1764705882352942No Hit
GTTCTGGTGAGTGGGAGTGGCTGCTGAGATGATCCCGATAACACAAGAGA11.1764705882352942No Hit
GTTCAGAAACATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGG11.1764705882352942No Hit
GTCTTTGCACATGGCTCATCTGCTTTCAGCCTGGAATCATTTGATTCCCT11.1764705882352942No Hit
GCCTGGTGCACCCCAGCTCAACCCGATGGATGGACCACTACCTGAGGATA11.1764705882352942No Hit
AAAGACGACAACCACGTTTAAGGGATGGAACTCCTACTCTAATGTGCATC11.1764705882352942No Hit
CAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGGAGG11.1764705882352942No Hit
GGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAA11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATT50.0145.04
TGCCGCA50.0145.0145
CACAAGA50.0145.02
ACACAAG50.0145.01
TTCCCCT50.0145.09
AAGATTC50.0145.05
GATTCCC50.0145.07
ACAAGAT50.0145.03
ATTCCCC50.0145.08
AGATTCC50.0145.06
Produced by FastQC (version 0.11.5)