FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGAAGCCGGGCTTTGCTTTATAGAGATGGCCTTCTGTTGTTTCGCTT17.142857142857142No Hit
TATCTTAGTTTAGCAACTTAGGATTTCAAATGCCTGATTTTTGAGGGAAG17.142857142857142No Hit
AGGACAAACTAAAAAGATACTTCGCCCTTAAACCTCCTTAGGTACCTTAA17.142857142857142No Hit
CATCATGTAAATCTACCTGAAGAACAATGGCGACTTCATCCTCGTGGTCG17.142857142857142No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA17.142857142857142No Hit
ATCCGGTCCCTAGACACCATGGCCGCCACCAGGCTAGAAATTACAACCGG17.142857142857142No Hit
ATTATGTGCAGACCCAGCTTTACATGTGCGGCGATATGCTCAGCAACGTC17.142857142857142No Hit
GTCAATTGCTTAGGAAATAATTGTGTCCAGGGTAGTATAAAATGAGGATT17.142857142857142No Hit
CCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTC17.142857142857142No Hit
ACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTT17.142857142857142No Hit
CACTACGTCACCTAGCCAAATTAGCCCATTAGCCTTCACACCTTTTCCAA17.142857142857142No Hit
GTGGGAGGAGCTTGATTGTGGGCACAAGGGAGCTTAATGTCGGCCAGATC17.142857142857142No Hit
TAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGACATCCG17.142857142857142No Hit
CATTGCCACAACTAATTCAGTGCATGTGTGAGGAACGAGTTGAACCAAGA17.142857142857142No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)