FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA30.2982107355864811No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.2982107355864811No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA30.2982107355864811No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.2982107355864811No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC20.19880715705765406No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG20.19880715705765406No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20.19880715705765406No Hit
GGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTCACTTCTT20.19880715705765406No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20.19880715705765406No Hit
ATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAA20.19880715705765406No Hit
GAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTA20.19880715705765406No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA20.19880715705765406No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20.19880715705765406No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCCTCAA20.19880715705765406No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.19880715705765406No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA20.19880715705765406No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.19880715705765406No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.19880715705765406No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.19880715705765406No Hit
TGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTG20.19880715705765406No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.19880715705765406No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC20.19880715705765406No Hit
GTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAA20.19880715705765406No Hit
GTCTTATAGTGAAAAGCCTAGTTTTGATTCTTGCCCTGCTCTCTTCGTCA20.19880715705765406No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATT20.19880715705765406No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT20.19880715705765406No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA20.19880715705765406No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)