FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGAGACTTGGCCCTTAAGGGTGAGGCCGCTCATCCGTACTGAGCA28.695652173913043No Hit
GGCTTAATTTGGAGCTAGAGCAGAGGGTCTGAACAAGAGAGCCAGACCGT14.3478260869565215No Hit
CGTATCAGAAGGTACTCATCTCCTCCCACCCCAGCATTTGTCCACATGGT14.3478260869565215No Hit
CTGTGAATGGGGTTCCTCGTCTGTGAAATGGGGGTAAGGGCTCTTATCAC14.3478260869565215No Hit
GTGTGGTCAGCTGTGGATGACTGGCTATGTATTCATTGTGTGCAAACGCT14.3478260869565215No Hit
GTTCTGTCAGGGCTGTGGAACACACCGAGACACGTGAGTCCCACTGCGTA14.3478260869565215No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT14.3478260869565215No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGCTCGTGAG14.3478260869565215No Hit
CTTCAATATAAACACTGACTGTTCCGCCGGCTACTGGGAGAAACCTTGTT14.3478260869565215No Hit
GGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACAGCAGG14.3478260869565215No Hit
ACTCAAGACTGACAGAATCCACATCTAATACCCAACACTGTAATTACTTT14.3478260869565215No Hit
AATTCACAGCTTGGAAGTGCTCTTGGAAGTGTCCCGAAGGAGGTCAGGAG14.3478260869565215No Hit
TGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCC14.3478260869565215No Hit
GCATAATGCCTGGAACCATCATCCATGGACATACTACAGAATTTCCATCC14.3478260869565215No Hit
GTTATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGAT14.3478260869565215No Hit
GCCCCATGCTTCTTAACATGGTGGTCTAATGGAGGAAACAAGAAAGCAAG14.3478260869565215No Hit
CCCTGGAACGGTGCCGGCAGCTGTGGTGGCGACGAGACCTGGAACGTGCC14.3478260869565215No Hit
CACTCAGACCCTACAATGAACCAGAACTCTCCGAGGGGTAGGGGAGATCG14.3478260869565215No Hit
TCCGTACTGAGCACCGGCTATGAGCGGGCCACTGGGCCAGGCACTTGGCG14.3478260869565215No Hit
CAGATGTAGGGGAGAAAGGGAAGCAGCTGGCTGCGGGACAGAGGCAGTGT14.3478260869565215No Hit
TGGTAGGCCCTTGTCATAAGTGCACCCGGAAAACTCGGAGCGAGAAGGGC14.3478260869565215No Hit
CAATAAAGGTTTATCAAAAAAACTCATGAAGTACCGCCTACCAGCCCTCT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)