FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_D01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_D01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019438
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15740.1543987961994746No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA15490.1519464646207028No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA11600.11378818525501307No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10450.10250745999266263No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC450.00966311448.3030133
CTATTCG5600.045.277419
AGCGAAA3500.043.5453531
TATATTC9000.041.8626063
CAATTCG2057.2759576E-1238.8723149
CAATATG10550.034.333589
CGAAAGC4450.034.192023
TCAATAT10650.033.329348
ATTATAT9350.032.60081
ATAGACC4100.031.80933
ATATTCA11550.030.7352644
AGTAGAA9850.030.2091261
TATAGAC9750.029.7482812
GTAGAAA11250.029.6753521
ATTCAAT12550.029.4364476
TAGACCT4000.028.9789624
TATACGT3251.8189894E-1228.9789624
TTCAATA12300.028.8583327
GTATATA6050.028.7903161
TATTCAA13150.028.6441885
Produced by FastQC (version 0.11.5)