FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTATTTATTTTAGAAAGAGGAGGCCTTGGTGAGAAGAGCAAAGGGAG13.0303030303030303No Hit
CTGTTGGTCGCTCTCTGGATGTCAGTAAGTATGCTAGAGTCCCTTTTCCG13.0303030303030303No Hit
GAGAACAACCCAGTTATTCTGCCAAAGGACCCAGCAGTGGCTGGGCTTCC13.0303030303030303No Hit
CCTCACAAGATATAGTTGGGTACCTAGAAGAACAGGACCCTCGTGTCCTC13.0303030303030303No Hit
GTATGCTAGAGTCCCGGTTCCCGTACATTACCAAAACCACCTGGCCTTGC13.0303030303030303No Hit
GGGGCTGCCGTGCTGCAGAATTTGTACCAGCCCACGGGGGGCCGGCGGCT13.0303030303030303No Hit
AGCCAACGCCCTCATGTGTGGCTGACCAAGCACAAAGTTGGCTAAGTACG13.0303030303030303No Hit
CCACAGATCAAGACCTGGCCAAAGGCAGACACTTAATGACTGAGGCACCC13.0303030303030303No Hit
CACCATGAGCCCTTACTTGGAAATAGCCTAGTCCTATCCTCACAAGGCCA13.0303030303030303No Hit
GTGCCAGGTGGGGGGTGGGGGAGCCTTGGCCCTGACGGGTGAGGCCGCTC13.0303030303030303No Hit
CACCAGCATGTTGGAGTCTACCAGAGAGCTGTTGATCTGCTTCAAGAACA13.0303030303030303No Hit
NNATGGGTGATGCTTCTAAGCCAGGACCCACACCAACCAGAACATAAGAA13.0303030303030303No Hit
CGGTTATAGAAACCGTTCTCAGGATTACCCTCGCTCACAGGTGAAAACTT13.0303030303030303No Hit
TTGTCCGCCTGTGGTCCTAGGAGCATTATTCTCAATACCCAAAAGGTGGG13.0303030303030303No Hit
GGACAAGTGGTGCGGGAGGCTGTTGGAGGGCTGCAGACGGTTCGCAGTTT13.0303030303030303No Hit
GCCCCTAGCCAAGCCGAGAAACCGTCAGAGGCTTGCCCCCCGGTCGTCGC13.0303030303030303No Hit
CAGAGGGGGTATACAGACTGCGCGGCTGTCACCAGGGGCCCACCCCCGCC13.0303030303030303No Hit
TGCAGTTACTGATGCTTAACTGTGGTGTGCAGCAGATCATGGCTGGGTCC13.0303030303030303No Hit
GCTCTCAGGGTGGTCATGTGCATGGTCATGGTCATGGTCAGCAGTGGCTC13.0303030303030303No Hit
ACTAAGGCCTATCACTGAGGAGGGAGCATTAGATGCAGAAAATTCACCTT13.0303030303030303No Hit
ACCAAAAGCAAGACCAGAACCAGAACGGTAACCAGGGCTAGAAGCGGAAC13.0303030303030303No Hit
TTTTTAAACTATTCGCCACTAGTTGATGGCCCTGCGAATTCTTGTGGTCG13.0303030303030303No Hit
GTATTAGGCCGCAGTACTCTGCAGCAGGGAAATGCGGCTCTTGGCAACAG13.0303030303030303No Hit
ATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGG13.0303030303030303No Hit
GTCTGAGAAGCCTCCCATTCACATCTTGTCTTTCACTGGAGAGCAGCTGG13.0303030303030303No Hit
AACCAGCACTGTTCGGTACCCGTGGGATTTCCAGTCCTGCAGAGACTGCT13.0303030303030303No Hit
GTCATCAATCAAGCTAATTACACTATCTCATCATCTGTTTATGCCTTTTT13.0303030303030303No Hit
ACCTTATAGGTCAAATGCTGCTTGATCTGATTTTTCTCTCCCGCAAGGAC13.0303030303030303No Hit
CCCGCTAGACAGAGGGACAGAGAGAGGGAGTTGTGCGAAAACTCTGCTTT13.0303030303030303No Hit
GTTTAAGGATTGTGATAAAGGCCCCACATTTCTTCCTTTTTAATCATAAC13.0303030303030303No Hit
CTTCAACTCAGTAAATGAATAATATTATAATGATAATTTGGACACCACTT13.0303030303030303No Hit
TTTTATGCCTAGTAGCAGGGGCTGAGTCTAGTTTCGGAACTCTGCGGACT13.0303030303030303No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)