FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824456
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG604.967144E-448.288714
AGCGATA450.00967377148.288718
AGCGAAA1900.045.786081
CGAAAGC2400.039.2345773
TATAGTA5000.028.980262
GCGAAAG3301.8189894E-1228.5411642
GTATTAC3709.094947E-1225.4710851
TAGTACA4900.023.6516154
GTATTAG3304.6909918E-821.968071
ATAGTAC5650.021.7940223
TATAATA6500.021.1778812
TTACGCA2857.1523646E-620.332099
CTAGTAC2205.938988E-419.7544733
GTATAAA4954.7475623E-1019.0389921
GTATAGG3451.7424863E-618.9116421
CCTACAC4253.304558E-818.7473833
ATATATG7350.018.7241953
CTATAGT3502.0159332E-618.6256474
TCCACGA1950.00686648718.5770912
GTACTAT4303.7416612E-818.5451371
Produced by FastQC (version 0.11.5)