FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284428
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCATACACAGGCCATCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCAT23880.8395797882065057No Hit
CTCTGGAACAGAGCAGACATACGGGTCCAGAGCAGTTGCCAGTCCCAGAA19840.6975403265501287No Hit
GTCTGTGGACGGAGTGGAGTAAGTGGTCTACGTGCGGGACGGAGTGCACC19560.6876960074254292No Hit
CTCTTAAGCAGCACGGCCACATACACCCTTACTTTTGAATGTCACCGTCC15880.5583135275008086No Hit
GTGGTACACTGAGAGATAAAAAGAGTGATAGAGCGAGGGACTGAGGGAAA15280.5372185579478814No Hit
GTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCTCTCTCTCTCTCTCT14870.5228036620867145No Hit
GCCACATACACCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTA13850.48694221384673797No Hit
AGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCTGCAATGAA13690.481316888632624No Hit
GTTGAGTAGGTGGGTGTGTCAACTCACTCTGCATACACAGGCCATCTGTG11450.40256233563502897No Hit
GAGCAGACATACGGGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGC9980.35087966023035705No Hit
GTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGT9750.34279325523506826No Hit
CATACACCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTT9580.3368163471950722No Hit
CTCCTGCAGTGGACGGTCTGTGGACGGAGTGGAGTAAGTGGTCTACGTGC9460.33259735328448675No Hit
GCATACACAGGCCATCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCATCG9000.3164245432939092No Hit
GAGTGGAGTAAGTGGTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAG8980.315721377642145No Hit
GTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGTCTTCTTCTCTTG8870.3118539665574416No Hit
GATCTGGGAGATGCAGTCTTTCTCTTAAGCAGCACGGCCACATACACCCT8850.31115080090567737No Hit
CATTAAGTGAGAGTCCATCCTCACCAGCAGAGTTGTCTTCTGCCACCAGT8570.30130648178097796No Hit
GTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGG8450.2970874878703925No Hit
GTGTGTGAGTGTGTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGT8400.2953295737409819No Hit
GGGTTAGGGTGATGGCCAGCAGTGGATCTGGTTGACCGTCTCAGTTGTAA8390.2949779909150998No Hit
GATGGAGAGAATGAGGGATGAAAACTACAGATGGACAGATGGAGAGAATG8220.28900108287510373No Hit
GATATGACATCTCCCTTTCCCTCAGTCCCTCGCTCTATCACTCTTTTTAT7910.27810201527275796No Hit
CTCTATCACTCTTTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTG7370.2591165426751234No Hit
GTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGT7240.2545459659386558No Hit
GCTATGAGCAATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGCCG7020.24681114376924915No Hit
GTGTATGCAGAGTGAGTTGACACACCCACCTACTCAACTGCACAAACAAA6910.24294373268454583No Hit
GCCTAATTGCAGATTGGGTCCCAATACCCACACGCCCTGACCCTCAAGAC6800.23907632159984252No Hit
CCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTTAGCCTA6740.23696682464454977No Hit
CCCTTTCCCTCAGTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTAC6730.23661524181866767No Hit
GTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAG6580.23134149943043583No Hit
CCCTGACCCTCAAGACAACTTGGGATCTCTGGAACAGAGCAGACATACGG6490.22817725399749672No Hit
GTGTGAGTGTGTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGTCT6460.22712250551985036No Hit
GTGTGTGAGTGTGTGTGATCATCGTCCCCTACCCTGGAGTCTTCTTCTCT6410.22536459139043977No Hit
ATTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCTGC6270.22044243182809006No Hit
GTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTGCCCCCATTCTTGG6180.21727818639515095No Hit
GTGTATATGCATGTATGTGTAGATGACCATATAGAAGTAGGAGTTTGAAT6180.21727818639515095No Hit
AGATATGACATCTCCCTTTCCCTCAGTCCCTCGCTCTATCACTCTTTTTA6170.21692660356926885No Hit
GTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTTGAACCCACA6000.21094969552927278No Hit
GTCTTTCTCTTAAGCAGCACGGCCACATACACCCTTACTTTTGAATGTCA5920.2081370329222158No Hit
GTCTGGAACTATCAGGTATGCTTTGCTTGCTAGCATGACTGATGGCTGTC5800.20391803901163036No Hit
GTGTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGT5730.2014569592304555No Hit
GAGTAGGTGGGTGTGTCAACTCACTCTGCATACACAGGCCATCTGTGCAG5730.2014569592304555No Hit
TTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCCCCTGCA5560.19548005119045947No Hit
GTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGT5500.19337055423516672No Hit
GCTTAAGTTAGCCTAGAGTTCTGTGTCTGGTCTGGAACTATCAGGTATGC5460.19196422293163823No Hit
CTACTAGGGTGAGTATCACAAGACCAAATACTGAGTTGCTATCAGTGAAT5390.18950314315046338No Hit
GCAGTGGACGGTCTGTGGACGGAGTGGAGTAAGTGGTCTACGTGCGGGAC5370.18879997749869915No Hit
GTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGTGTCA5200.18282306945870308No Hit
CTACAGTAGTGTTCAGGATTTCATAGTTTCATACATCTGGCCATACTTGA5100.17930724119988187No Hit
GTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAGGCGTGAGTGTAATG4820.1694629220751825No Hit
CTTTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCT4750.16700184229400764No Hit
GTGGGGGCACTGTCCACCTGCTCCGCCTCTATGCTCTTCTCGCTCAGGCC4720.16594709381636127No Hit
GTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGTGT4680.16454076251283278No Hit
TCCCAGAACAGCAATTGCCCCTTGAACCCACATTAAGTGAGAGTCCATCC4640.1631344312093043No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTTGA4530.15926702012460095No Hit
GTCTGAGGCTGGTTGAAGGGGTTAGGGTGATGGCCAGCAGTGGATCTGGT4510.15856385447283672No Hit
GTGCAGTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTGCCCCCATT4360.15329011208460488No Hit
GTCCCAATACCCACACGCCCTGACCCTCAAGACAACTTGGGATCTCTGGA4340.15258694643284065No Hit
GCCTAGAGTTCTGTGTCTGGTCTGGAACTATCAGGTATGCTTTGCTTGCT4340.15258694643284065No Hit
AGCTTAAGTTAGCCTAGAGTTCTGTGTCTGGTCTGGAACTATCAGGTATG4330.15223536360695852No Hit
GTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTACCACCTCCTCAGT4310.1515321979551943No Hit
GAGCAGAGGTGAGTGAGTGCAAGTGGCTGTTTGAGGTAACTGCGTCAGTC4300.15118061512931216No Hit
GTGATAGAGCGAGGGACTGAGGGAAAGGGAGATGTCATATCTCCTCCCCC4160.1462584555669625No Hit
GTGTGTGTGTGTGTGAGTGTGTGTGAGTGTGTGTGATCATCGTCCCCTAC4130.1452037070893161No Hit
GAATGAGGGATGAAAACTACAGATGGACAGATGGAGAGAATGAGGGATGA4020.14133629600461278No Hit
GGGGAGGAGATATGACATCTCCCTTTCCCTCAGTCCCTCGCTCTATCACT3980.1399299647010843No Hit
GAGTGCACCCACTGGCGGAGGCGTGAGTGTAATGCCCCCGCGGCCAAGAA3970.13957838187520216No Hit
ACCAAATACTGAGTTGCTATCAGTGAATTTGACAGTGAGAGTGACTTATC3830.13465622231285246No Hit
CTTCTGGAGGATCCCAACTGTAAACTGGAGCTACTAGGGTGAGTATCACA3800.1336014738352061No Hit
GTGTAATGCCCCCGCGGCCAAGAATGGGGGCAAGGACTGCGATGGCACCC3770.13254672535755974No Hit
GGACTATTATTAGTCTGGACTGTGGATCACTGCTATGAGCAATGTCGCCC3700.13008564557638488No Hit
TTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTGCCTTCTCTCTCT3700.13008564557638488No Hit
GTTCTGTGTCTGGTCTGGAACTATCAGGTATGCTTTGCTTGCTAGCATGA3660.1286793142728564No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGCTTGTTTGTTTGTGCAGTT3600.12656981731756367No Hit
GAGCAATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGCCGGAGCC3590.12621823449168154No Hit
CTCTCTCTCTCATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGGAGTG3560.12516348601403518No Hit
GTAGGTGGGTGTGTCAACTCACTCTGCATACACAGGCCATCTGTGCAGTT3520.1237571547105067No Hit
ATATAAGCGTGCCCTCTCAGTATTCTGCATAGCAACAAGGCAAGAACCAA3510.12340557188462457No Hit
CATACATACTGTGATCCGCTCATACACAGATTTGCACACAAATATTCAAA3480.12235082340697821No Hit
TACATACATACTGTGATCCGCTCATACACAGATTTGCACACAAATATTCA3470.12199924058109611No Hit
ACCCTGGAGTCTTCTTCTCTTGACCGGGTGGGAGAACACACCTGTTTCCC3450.12129607492933185No Hit
GTATGGGAGCACGGAGGTGTATAATTCTGTAGGTCACTCTGCCCTTGCTC3440.12094449210344972No Hit
ACTGATGGCTGTCTGAGGCTGGTTGAAGGGGTTAGGGTGATGGCCAGCAG3400.11953816079992126No Hit
CTTAGGGCCGGAGCCATGTTCTGAATACCAAGCACTTCTCATAAAAGGAG3380.118834995148157No Hit
TACGGGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTTGAAC3380.118834995148157No Hit
GGCCATCTGTGCAGTTCTTGGACTGGAGCAGGGTGCCATCGCAGTCCTTG3370.11848341232227488No Hit
CATACATACATACTGTGATCCGCTCATACACAGATTTGCACACAAATATT3360.11813182949639277No Hit
AGGTGGTACACTGAGAGATAAAAAGAGTGATAGAGCGAGGGACTGAGGGA3340.11742866384462851No Hit
CCCAATACCCACACGCCCTGACCCTCAAGACAACTTGGGATCTCTGGAAC3320.11672549819286428No Hit
GGTATGCTTTGCTTGCTAGCATGACTGATGGCTGTCTGAGGCTGGTTGAA3310.11637391536698215No Hit
AAGCAGGAGAGAATGAGGGATAAAAACTACAGATGGACAGATGGAGAGAA3290.11567074971521792No Hit
GTCTAGACTGGGCTAGAGTGTGTCAGTGGTGGGCGGGCCCTCACAGCGCG3280.11531916688933579No Hit
GGAGAGAATGAGGGATAAAAACTACAGATGGACAGATGGAGAGAATGAGG3270.11496758406345367No Hit
CTATCACTCTTTTTATCTCTCAGTGTACCACCTCCTCAGTCTTTTCTGCC3260.11461600123757155No Hit
ATGTCGCCCCCTGCAATGAATCATGGGGCTCTTAGGGCCGGAGCCATGTT3250.11426441841168943No Hit
GAGTTACACCACCAAGCCAGGAGTTACAACTGAGACGGTCAACCAGATCC3250.11426441841168943No Hit
GCAGGAGGGGGAGGAGATATGACATCTCCCTTTCCCTCAGTCCCTCGCTC3250.11426441841168943No Hit
GACATACGGGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTT3220.11320966993404306No Hit
CCTCAGTCCCTCGCTCTATCACTCTTTTTATCTCTCAGTGTACCACCTCC3210.11285808710816093No Hit
CTATTAATCCACCAGTAAACACTTCTTATCACTCTCATTTTCCATCTTTA3190.1121549214563967No Hit
CTCTCTCTCTCTCTCATCTCCCCCTCCTGCAGTGGACGGTCTGTGGACGG3190.1121549214563967No Hit
GTCCCAGAACAGCAATTGCCCCTTGAACCCACATTAAGTGAGAGTCCATC3140.1103970073269861No Hit
CCTCAAGACAACTTGGGATCTCTGGAACAGAGCAGACATACGGGTCCAGA3140.1103970073269861No Hit
GGGCTAGAGTGTGTCAGTGGTGGGCGGGCCCTCACAGCGCGCTGCCAGCC3130.11004542450110397No Hit
TCTCTTGACCGGGTGGGAGAACACACCTGTTTCCCCTCTGCCTTTTTTTT3110.10934225884933972No Hit
CATCTACAGTAGTGTTCAGGATTTCATAGTTTCATACATCTGGCCATACT3060.10758434471992911No Hit
GTGGATCACTGCTATGAGCAATGTCGCCCCCTGCAATGAATCATGGGGCT3050.107232761894047No Hit
CGCCCTGACCCTCAAGACAACTTGGGATCTCTGGAACAGAGCAGACATAC3040.10688117906816488No Hit
GAATGAGGGATAAAAACTACAGATGGACAGATGGAGAGAATGAGGGATGA3020.10617801341640062No Hit
GTGTATGTGTGATTAAGTAAGTGTGTGTGTGTGTGTTTGAGAGTGCTTGT2990.10512326493875426No Hit
GATAAAAAGAGTGATAGAGCGAGGGACTGAGGGAAAGGGAGATGTCATAT2990.10512326493875426No Hit
GTCAACTCACTCTGCATACACAGGCCATCTGTGCAGTTCTTGGACTGGAG2950.10371693363522577No Hit
CTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTTAGCCTAGAGTTC2930.10301376798346154No Hit
GGAGTAAGTGGTCTACGTGCGGGACGGAGTGCACCCACTGGCGGAGGCGT2910.10231060233169731No Hit
GTCCTTGCCCCCATTCTTGGCCGCGGGGGCATTACACTCACGCCTCCGCC2900.10195901950581518No Hit
GGTCCAGAGCAGTTGCCAGTCCCAGAACAGCAATTGCCCCTTGAACCCAC2880.10125585385405092No Hit
GTGTTTGCTTGTTTGTTTGTGCAGTTGAGTAGGTGGGTGTGTCAACTCAC2870.10090427102816882No Hit
CACCCTTACTTTTGAATGTCACCGTCCCGGTGACCAAGCTTAAGTTAGCC2850.10020110537640456No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT100.0070825145.03908145
TGGCCTA100.0071048946144.886035
GGCCTAA100.0071048946144.886036
ACTTTGC203.8779955E-4108.645429
CAGTCCG352.7521855E-7103.599335145
CACGGGG259.3697425E-487.023445145
AGCGAAA2250.083.888981
GGCTACT353.410515E-582.967121
AAGGACG300.001932243672.51954145
TTAAGCC300.001939001672.4557654
GCGAAAG2750.068.588072
GCCTAAT1400.067.410781
GTCCTGG350.003545194662.2253381
GCTTACT350.003555108362.18152
CTTACTG350.003567529662.1267933
GGTGACT350.00357499862.0940135
ATACGCT1550.060.7693444
GGACTAT1450.060.079641
CTAATTG1600.058.891023
GACTATT1500.058.036072
Produced by FastQC (version 0.11.5)