FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840807
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1900.053.4495351
CGAAAGC2050.049.479653
TTATCCG450.00967503748.2871979
GCGTAAC2401.5716068E-930.224441
CCGACGC1606.8159294E-527.193897145
GCGAAAG4001.8189894E-1225.3703962
CGTAACA3508.7486114E-820.7105272
TTACGCA2806.138889E-620.6945134
ATTACGG2155.087863E-420.2132475
CATTACG2155.087863E-420.2132474
GTAATAC3259.888063E-720.0637263
GTTACGC2908.293911E-619.9868553
GTATTAG4051.8693754E-819.7018571
TAATAGC4051.8757419E-819.695992145
TATTACA5555.456968E-1219.591042
GTATTAC4102.1522283E-819.461591
GTATAAT3451.732471E-618.9231281
GTATATA5056.148184E-1018.6733191
CGTCGAA551.2411602E-418.45888770-74
CTATAGA3552.3094944E-618.3627384
Produced by FastQC (version 0.11.5)