FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTAGGCTAATTCATTAATGTGAAACAGGCTCATATTTTCAGACTAAT50.33967391304347827No Hit
GCATAAATGTTATTTGCTCAAAACTACTCTCTGTTATCTTCAATTTATGT30.20380434782608695No Hit
CTCCAGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTAACAATAACT30.20380434782608695No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT30.20380434782608695No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC30.20380434782608695No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC20.1358695652173913No Hit
GTATTGGCCTCTAATGGCCTTAGGAACTAGAGACTGAAATGGCTCAAATT20.1358695652173913No Hit
GGTCTATCCCTACCAAAGAGTATCCCTCTTTTTCGAAGTCGTACCCACTG20.1358695652173913No Hit
CCTTTAATTTCAATGTTTGCAGATTGCCCGTAAGCACTTCTTCCTCTCTC20.1358695652173913No Hit
GTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATT20.1358695652173913No Hit
GTATTCTCACAAGAAGATTGTATGATAAAGGCAGTTAGAGGTGATCTGAA20.1358695652173913No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA20.1358695652173913No Hit
GTTTTATTATCTGGGTGGTGTCAAAAGTTCCAAGCACATCCCTCATTTGT20.1358695652173913No Hit
ACAGAGGACTCAGCTTCAATCATACTCTCTATTTGTTGAAGTGATTGGAG20.1358695652173913No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA20.1358695652173913No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG20.1358695652173913No Hit
GCCAGGTAGGCCGCGGTGTGTGCGCAGAGCGGATCGAAGTGATGAATGGT20.1358695652173913No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.1358695652173913No Hit
GATTATTAGGTGCCACTTAGTCTGCATATGTACAAGAGCATGTTTAGACC20.1358695652173913No Hit
GATATGAATAAAGGGACTAGGTTTGTTCTTGTACAAGAAAAAGAGCTAAT20.1358695652173913No Hit
GAAATATTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGGCGAGA20.1358695652173913No Hit
GATCAGAAGTCCCTAAGGGGAAGAGGCAGCACTCTCGGTCTGAACATCGA20.1358695652173913No Hit
GCTGACAACCTCCTCAAATCCCACAGCAGTGGCACCTACCTGATCAGGGA20.1358695652173913No Hit
GTACTGTTCACTACCCAAAGATCTACAAAACTTATTTTGAAAAAGTCGAA20.1358695652173913No Hit
CCGTTTACACAGACACATTCAGACTCTTGTGTTCTTAATATTCGCTTCCT20.1358695652173913No Hit
GCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTC20.1358695652173913No Hit
ATGTGAAACAGGCTCATATTTTCAGACTAATATTTTAAGCCTACCAATGG20.1358695652173913No Hit
ATGTGATGGGAATGATTGGGATATTACCCGACATGACTCCAAGCACCGAA20.1358695652173913No Hit
GAAGTGGAGGAAACACTAATCAACAAAGGGCCTCTGCGGGCCAAATCAGC20.1358695652173913No Hit
CAATTAGACTTCCGGATGGGCCTCCTTGTTTTCAGCGGTCAAAATTCCTG20.1358695652173913No Hit
GCAATGAAGAGAGGAAGACATGAAAGCGACTGAGTGATATGCAATTAAGT20.1358695652173913No Hit
CATATTATGTGTTCAATCCCTGGGAAGATAAAGTTAAAAAGATACATTCC20.1358695652173913No Hit
GTCTCTTAGTGGTCTTGTTGTAATTGAATACTGGAGAATTACCTCTTACA20.1358695652173913No Hit
GTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCT20.1358695652173913No Hit
ACCGTAGACCACATGGCCATAATCAAGAAATACACATCAGGAAGACAGGA20.1358695652173913No Hit
GTACTTAACTGTAAGAACTAATAAGAAGCCTGCATTAAGAGATGTTAAAA20.1358695652173913No Hit
CTGAAGGAAGAATCCGATGAGGCACTTAAAATGACCATGGCCTCCGCACC20.1358695652173913No Hit
GCTGTCATGGAAGCAGGTACTGGCAGAACTGCAGGACATTGAGAGTGAGG20.1358695652173913No Hit
GCTGTCCTATAGGTGAAGCTCCGTCTCCCTACAATTCAAGGTTTGAATCA20.1358695652173913No Hit
GACAGAGACATTGCATTGTGATAAAGATGACAATGTGTAGGCTAATTCAT20.1358695652173913No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT20.1358695652173913No Hit
CATAAGGCAGTGGCCTCCATTGGAGAAGGCCTTGCCAACGTGGAGCTGAA20.1358695652173913No Hit
ATACACCAGTGTGTTTACCAATAATGAGGACACAGGGACTTTTTCAGTTT20.1358695652173913No Hit
GGTAGATTGCTTCCTTTGGCATGTCCGCAAACAAGTTGCAGACCAAGAGC20.1358695652173913No Hit
CCTCCATAGTGCTTTCTTGTAAAATCTGAGAAAGAATATCTAAGAATCCA20.1358695652173913No Hit
TACTGACTCAGGGCCATTAATCTCCCACATCATTGATGAAGAGTAAGTTA20.1358695652173913No Hit
GATGTATCCTATTTGATAATCCAGGTTTTGATTAAAAGACACCCAAGGTC20.1358695652173913No Hit
GTCATAAAGGACAAGTTCCCTCATCCACTTTCCATCTACTCTCTTGTATA20.1358695652173913No Hit
ACATAAAACACTCAAATACAGCAATGTAATGTAATTATAATAAATTGCCA20.1358695652173913No Hit
GTGATAAAGATGACAATGTGTAGGCTAATTCATTAATGTGAAACAGGCTC20.1358695652173913No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.1358695652173913No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAG100.0063819443145.03
GTGGTCG100.0063819443145.0145
Produced by FastQC (version 0.11.5)