FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563393
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC13680.24281451846224572No Hit
GTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATT10660.18921072856780258No Hit
GTGATAAAGATGACAATGTGTAGGCTAATTCATTAATGTGAAACAGGCTC8810.15637396985763047No Hit
GTGTAGGCTAATTCATTAATGTGAAACAGGCTCATATTTTCAGACTAATA8300.14732167421320463No Hit
AAGCAGGGACGTGCACAGGAATTTTGAAGGGCAAAACAAAAATCACACAG8100.1437717543526455No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC7920.14057682647814226No Hit
GTATGGAGAACTGAGAAGAACAGGGTCTTTATTAATAGTAATACATAAAA7590.13471945870821966No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6470.11483990748908843No Hit
GACAGAGACATTGCATTGTGATAAAGATGACAATGTGTAGGCTAATTCAT6420.11395242752394864No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG6240.11075749964944541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCC253.709465E-8144.99937145
TTGACGT100.0071052113144.896419
AGCGAAA1850.098.077091
GAGCGTT259.407967E-486.9455644
AGCGTTG259.411285E-486.937845
CGTTGAG259.411285E-486.937847
GCGTTGA259.411285E-486.937846
GGGTAGA451.18714954E-464.512931
TAAACTA1850.062.6579065
CGAAAGC3050.059.410143
AGAACGG2300.056.6985975
CTATTCG2050.056.544949
GTGAGAC902.05182E-856.3736233
AACACTG4250.056.25395
GTGCGTC651.0976644E-555.7293979
TCCCAAC4300.055.6986661
TCAACGC400.00604962154.374763145
GGAGCGT400.00605600254.360283
TTTAAAC2200.052.7129973
ACGGCCC2350.052.409348
Produced by FastQC (version 0.11.5)