FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG50.29291154071470415No Hit
GCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAGCACTCAGTT30.17574692442882248No Hit
ATCTAAGAGTATCAAGCTTCATCAGAGGGACAAGAGTACTTCCAAGGGGG30.17574692442882248No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC30.17574692442882248No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA20.11716461628588166No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA20.11716461628588166No Hit
ATGCTGGCCCATATCTCCTATCTTCTTTGCCCAGAATGAGGAATCCCCTT20.11716461628588166No Hit
CCCAAGGTCGATTCGAACCATGCCAATTGTCTCTGCACACACACATCACT20.11716461628588166No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT20.11716461628588166No Hit
GCCTCATACAATCCAATCCTGTTAGTTCAGGATGTTGAACGAAGCTTCCG20.11716461628588166No Hit
CTCATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCT20.11716461628588166No Hit
TTGTGCAGCTGTTTGAAATTTCCCTTTGAGAATGTTGCACATTCTCTCAT20.11716461628588166No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA20.11716461628588166No Hit
CTATTAAATGACAAGCATTCAAATGGAACCGTTAAGGACAGAAGCCCTTA20.11716461628588166No Hit
GTACTGTTCACTACCCAAAGATCTACAAAACTTATTTTGAAAAAGTCGAA20.11716461628588166No Hit
GTTCAATTCCCCAATTTTGGAAAAGCACTTTAGCATCCTTCTGAAAATGC20.11716461628588166No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC20.11716461628588166No Hit
CAGTTAGAGGTGATCTGAATTTCGTTAATAGAGCGAATCAGCGGTTGAAT20.11716461628588166No Hit
ATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGTGGAGATGCATACAGA20.11716461628588166No Hit
ACGTGACAGTGACACACTCTGTCAACCTACTTGAGGACAGTCACAACGGA20.11716461628588166No Hit
GTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGCTGACAAAATGACCA20.11716461628588166No Hit
GTCAAGGAGAAAGACATGACAAAAGAGTTTTTTGAGAATAGATCAGAAAC20.11716461628588166No Hit
ATCCCAATGATACTTGCGGCAACAACAAGAGGATCACTTGAATCGTTGCA20.11716461628588166No Hit
ATGTATAGGCTACCATGCGAACAACTCAACCGACACTGTTGACACAGTAC20.11716461628588166No Hit
TCTCATTACTGCTTCTCCAAGAGAATCTCTGTAGAGTTTTAGAGACTCGA20.11716461628588166No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCTAAGAG20.11716461628588166No Hit
GAACAGAATGGTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGG20.11716461628588166No Hit
CAATTAACGATAACCAAAGAGAAGAAAGAAGAACTCCAGAATTGCAAAAT20.11716461628588166No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC20.11716461628588166No Hit
GGGTAAATCAAACATATGTTAATATCAACAACACTAATGTTGTTGCTGGA20.11716461628588166No Hit
GAGTAAAGGGGTTTTCATACAGGTATGGTAATGGTGTTTGGATAGGAAGG20.11716461628588166No Hit
GTTATCTCCCTCTTAAGCTTTCTATACAGTTTAACGGCTCTGTCCATATT20.11716461628588166No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA20.11716461628588166No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA20.11716461628588166No Hit
CTCTCTATCGTCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT20.11716461628588166No Hit
CCTTAAACGCGGTCTGAAAAGAGGGCCTTCTACGGAAGGAGTACCAGAGT20.11716461628588166No Hit
CGCCGGCACAGTGATGTGTGTGTGCAGAGACAATTGGCATGGTTCGAATC20.11716461628588166No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT20.11716461628588166No Hit
ATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAA20.11716461628588166No Hit
GTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAACTGACCCTCTT20.11716461628588166No Hit
CAATAAAATGGAATTTGAGCCATTTCAGTCTCTAGTTCCTAAGGCCATTA20.11716461628588166No Hit
CCTATCGACAATGTGATGGGAATGATTGGGATATTACCCGACATGACTCC20.11716461628588166No Hit
GGCCATGGAGGTTGCTAGCCAGGCCAGGCAGATGGTGCAGGCAATGAGAG20.11716461628588166No Hit
GAATTAATATGAGCAAAAAAAAGTCTTACATAAACAGAACAGGTACTTTT20.11716461628588166No Hit
TATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGTAT20.11716461628588166No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT20.11716461628588166No Hit
CCTCCAATCACTTCAACAAATAGAGAGTATGATTGAAGCTGAGTCCTCTG20.11716461628588166No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT20.11716461628588166No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.11716461628588166No Hit
GCACTCATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTG20.11716461628588166No Hit
AGCCAGCCAAGTGTACAGCCTAATCAGACCAAACGAGAATCCCGCACACA20.11716461628588166No Hit
GGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAACATTCC20.11716461628588166No Hit
CTACCATACTGGATATTCCAACTGGTCTTCTGTATGAGCTGCTGGGGAAG20.11716461628588166No Hit
ATTATATTCAATATGGAAAGAATAAAAGAGCTAAGGAATCTGATGTCGCA20.11716461628588166No Hit
CTCTTAAGCTTTCTATACAGTTTAACGGCTCTGTCCATATTATTTGGATC20.11716461628588166No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA20.11716461628588166No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)