FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGAGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGA11.639344262295082No Hit
TCCGTAGCCAGACTACAACTGAGGCCCAAGCCGTAGACAGATGCAAGGCA11.639344262295082No Hit
GATTCAAGCTGGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGAT11.639344262295082No Hit
GCCATAAGGTCCAGGAGTGGAGGAAATACCTATCAACAAAAGGCATCCGC11.639344262295082No Hit
GTAAGTAAGTATGATAGAGTCCAGTTATAGTTTAATTTCCAAAACTACTG11.639344262295082No Hit
CCATTAATAAGACATGAGAACAGAATGGTTCTGGCCAGCACTACAGCTAA11.639344262295082No Hit
GCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAA11.639344262295082No Hit
CCGTGTGAAGTGGGCACTCGGGGCAACTATGGCACCTGAAAAAGTTGCCT11.639344262295082No Hit
GTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGA11.639344262295082No Hit
TCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCA11.639344262295082No Hit
AGGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAATTTCAAAC11.639344262295082No Hit
GTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGCACAAAGAGCAATGA11.639344262295082No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT11.639344262295082No Hit
GTTTTCATTTGAAGCAAATACTACTCCTCTAGAGGAAAGCTTCCCCCTGG11.639344262295082No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC11.639344262295082No Hit
ATCTTGCTTTATTGAGAAGTTATAGTCTGTCCAAGACCATCCATTCGGAT11.639344262295082No Hit
GCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAA11.639344262295082No Hit
GTATGGTTATCATCATCAGAATGAACAAGGATCTGGCTATGCTGCGGATC11.639344262295082No Hit
TCCCAACACTGTGTCACGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC11.639344262295082No Hit
CCCAGGGCCCACGAAGATGAAGAGGGCAAGCCAATTGAAGGGGGCACTCG11.639344262295082No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT11.639344262295082No Hit
CATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCAC11.639344262295082No Hit
GTTGATTAGTGTTTCCTCCACTTCTGGTCCTTATGGCCCAGTACCTGCTT11.639344262295082No Hit
GCTAAAGACAAGCCCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT11.639344262295082No Hit
TTCCTTGATCGGCTTCGCCGAGATCAGAAGTCCCTAAGGGGAAGAGGCAG11.639344262295082No Hit
ATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGGCT11.639344262295082No Hit
ATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGA11.639344262295082No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGGCTAGCACC11.639344262295082No Hit
GATCCCAGGATGTGCTCTTTGATGCAGGGTTCAACTCTCCCTAGGAGATC11.639344262295082No Hit
GTTTCTCGGCCCAGCAACCGTAGGTTGTATGCTGATTTGGCCCCCAGCGG11.639344262295082No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11.639344262295082No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT11.639344262295082No Hit
GATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGA11.639344262295082No Hit
ATGTATAGGTTTTGATGCGAAAGATTCAACAGACACTGTAGACACAGTAG11.639344262295082No Hit
GATTAGGCTGTACACTTGGCTGGCTTGAAGCAGTTTGAAAGGGTCTATCC11.639344262295082No Hit
GTATGGTTCGCATTGAGTGCGGTTGAATGCAGACGAGGCGTGGCTGGTGG11.639344262295082No Hit
GCCCAGTCCTGCGGGTTAAGGGTCACGAGGACGATTCGTGAAGCCTAATG11.639344262295082No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT11.639344262295082No Hit
CTCTAGCTCTATGTTGACAAAATGCCCATCGTCAACATCCACAGCACTCT11.639344262295082No Hit
TCAGTGTGCAGCCTACATTCTCAGTGCAGCGGAATCTCCCTTTTGAAAGA11.639344262295082No Hit
GCCCTACCGTTTATTGGGTTTGATCCCCCAGAGTCTTTAACTCAAATGCT11.639344262295082No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG11.639344262295082No Hit
AGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATT11.639344262295082No Hit
CCAGCATGCACCTGAAATCAGAGCATCCGGCGGAAAAATGCTTGGTGGAA11.639344262295082No Hit
ACCTTATACTAGAGACGGACTGGTCAGCTTGCTGATGTGGGGTTAACAGG11.639344262295082No Hit
GCATCATATAGTGGTATTCCCTCCCCCTCGTGAGTCGGGGCTTCAATACG11.639344262295082No Hit
GGATACACCATGGACACAGTAAACAGCACACACCAATACTCAGAAAAGGG11.639344262295082No Hit
GACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAA11.639344262295082No Hit
GATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAG11.639344262295082No Hit
GGGTGGGGGGGGGGCGGTGGCGGGGCGGGGGCGGGGGGGGGGGGGGGGGG11.639344262295082No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC11.639344262295082No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA11.639344262295082No Hit
CAAGGGTATTTTTCATTAATTGTCGGACTCCTCTGCATTGTCTCCGAAGA11.639344262295082No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCATCATCATCAT11.639344262295082No Hit
GCAAATAGTAGCAGAGCAACATCTATCTCAAAGACAGAGTTAGGTAGGAG11.639344262295082No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG11.639344262295082No Hit
ACATTCAGATTATCCCAATGAGAATCAAAGGTAGTACCAAAGACGGAAGA11.639344262295082No Hit
AGTTTATCTACCCTTGTTTGTTGAACAACTTCCATTGTTTCAAGGCATGA11.639344262295082No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA11.639344262295082No Hit
GGACAAGAGTCCTTCCAAGGGGGAAGCTTTCCACTAGAGGAGTACAAAAT11.639344262295082No Hit
GTCATACTCCATATTTTTGGCTGGACCATGTGCCGGCATCATCACTGCAT11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA50.0145.02
CCTGATT50.0145.0145
GTCTGTA50.0145.09
TAGTCTG50.0145.07
TCAATAG50.0145.03
CAATAGT50.0145.04
AATAGTC50.0145.05
ATAGTCT50.0145.06
AGTCTGT50.0145.08
ATTCAAT50.0145.01
Produced by FastQC (version 0.11.5)