FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGAGGTGAGTCAATGATTCAGCAAATAACAAAAGAGAGAGAGTTTT13.4482758620689653No Hit
ATTATGTATTCAGGAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTT13.4482758620689653No Hit
GGGGCGATCTTAACTTTGTCAATAGGGCAAACCAGCGACTGACCCCCATG13.4482758620689653No Hit
ACACTGCCTGTTCCGCCGGCTACTGGGAGAAGCCTTGTTGTACGGCCCAA13.4482758620689653No Hit
ACACTAATAGATGCCCATCCGAATTCTTTTGGTCGCTTTCTGGCTGTCAG13.4482758620689653No Hit
GCCAAATGCCGATTCGGTGGTCCCAGCCCCCATCCTCTTGTATATGAGTC13.4482758620689653No Hit
GATGTAATGGAATCAATGAACTAAGAGGAAATAGAGATACCAACCCACTT13.4482758620689653No Hit
ATATCATGTTGTACTTTATCACTGTTACTCCAATACTCATGTCAGCTGAT13.4482758620689653No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG13.4482758620689653No Hit
AACCAATTGTCACAATAGGGATAGTGGGAGATAAACACTCAAACTCGTGC13.4482758620689653No Hit
GATATAAGTAATCATCGCAAGGAACATAAGAGGATTATGATTTTCATTCA13.4482758620689653No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG13.4482758620689653No Hit
CTGTACAGGCTTCTGGTTGCCGTAGGGACAAGAGACTGAAATGGTTCAAA13.4482758620689653No Hit
CTACTTCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACT13.4482758620689653No Hit
GTTGTAATTTCCATGATCAAGGTCGTGCAGAGTGCGGCCTTGTTCTCGTG13.4482758620689653No Hit
ATTAAACAGAAATCAACCGGCAGCAACTGCATTGGCCAACACCATAGAAG13.4482758620689653No Hit
CATTTGATAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC13.4482758620689653No Hit
GGCAAATGTTGTGAGAAAGATGATGACTAATTAACAAGCCACAGAGATTT13.4482758620689653No Hit
GTCGGATCTTGTGACAATTCAATTTTCACAATTTCCCATATCCAGATTAT13.4482758620689653No Hit
CCCTAAGGAAAAAAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTCC13.4482758620689653No Hit
CCCCCATACTATAAAATGGATCAATCTCCTGCTTGCCTATCTGAAAATAG13.4482758620689653No Hit
CCCATATCCTCTCCAGTCAATGAAAACATGTGAATGTGTGTCTTCTCAGA13.4482758620689653No Hit
GATTTTGCCAGTAACCTACTGCAAACTTTCCCAATAGAGCGTTCCTAAAC13.4482758620689653No Hit
GGCTTTAACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTT13.4482758620689653No Hit
ACTCTCTTCTGGTGGGGCAGCAGCAAAGGGGTGAAGTTTTATTATTTGGG13.4482758620689653No Hit
AATATAAACACTGCATGTACCGCCTGCTAATGGGAGAAACCTTGTTTTAC13.4482758620689653No Hit
CTTAAGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGAAGTGAA13.4482758620689653No Hit
GGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT13.4482758620689653No Hit
GAGTTGGAACAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATC13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)