FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_C01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_C01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859256
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG40640.47296731125531855No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA37880.44084649976258533No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA20270.23590175686873296No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT15200.1768972227136034No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC14640.17037995661362854No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG13970.16258251324401576No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG13220.15385403186012084No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT10820.12592289143165714No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC10750.12510823316916028No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT9950.11579785302633908No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG9750.11347025799063376No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC9660.11242284022456638No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA8800.10241418157103356No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT8780.102181422067463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATTC10450.056.836423
CTATTCG11600.053.6869169
ATTATAT10700.050.177711
ATATTCA11300.049.34184
ACTATTC13450.047.379278
TCAATAT11250.046.9892358
AAGGTCG6950.045.8454139
GTCAATT3650.045.684492
TTATATT12250.044.979162
TAAACTA15500.043.913585
CAATATG12500.043.4489449
TATTCAA12450.043.0392885
TTCAATA12350.042.8039557
TTAAACT16100.042.726814
ATTCAAT12750.041.458676
TATACGT1401.4295438E-841.377544
AAACTAT16300.040.8698436
AACTATT16950.039.732087
TAGAAAC12200.039.2209322
TTTAAAC19100.036.784723
Produced by FastQC (version 0.11.5)