FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCAACATTCTCATTTGAAGCAATCTGGACCCCTCTGGTGGAAAGAT19.090909090909092No Hit
GCTAAATACGGTATTGGGAGACTCGATACTGAATCTAGGACAAAATATCT19.090909090909092No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCCCCCAGGAATATTT19.090909090909092No Hit
GGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTGGACCATTGTT19.090909090909092No Hit
CCTATATGAACTGCTAGGGAAAAATTTCGCGAAGAGATGGCAGCACGTCT19.090909090909092No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC19.090909090909092No Hit
CAGATATACAAGTATAGTGAACAAGTGCTATAAAAGAAGAAAGCACGTCG19.090909090909092No Hit
GCTCGCAAGAGTCAATTGGTGTGGATGGCGTGCCCCTCTGCTGCATTTGA19.090909090909092No Hit
GTTTCCTTTGCCATATCCGCAAGCGATTTGCAGACAGTGGATTGGCTGTT19.090909090909092No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGC19.090909090909092No Hit
GTTTAGAGGTTGCCCGTTAGAACTTCTTCTTCTTTCTTGAATGATGATCC19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)