FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_B11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_B11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889560
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA79280.891227123521741No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT56030.6298619542245605No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT53270.5988353792886372No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG49300.5542065740366023No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA35170.39536400017986423No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC34590.38884392283825714No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT34230.3847969782813976No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30950.3479248167633437No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC29520.3318494536624848No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC29240.3287018301182607No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA27350.307455371194748No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG26980.30329601151130897No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG24710.2777777777777778No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG24610.27665362651198344No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG24230.272381851701965No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG23950.2692342281577409No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG23770.2672107558793111No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT22650.25462026170241464No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20990.2359593506902289No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20970.23573452043707002No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20680.23247448176626648No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20480.23022617923467784No Hit
CCCTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20370.22898961284230404No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG19730.2217950447412204No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC19440.21853500607041682No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG19120.214937722019875No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG18760.21089077746301543No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT18710.2103287018301183No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG18450.20740590853905302No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG18010.20245964296955798No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC17200.19335401771662394No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA16720.18795809164081118No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG16180.18188767480552184No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC16090.18087593866630694No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA15570.17503035208417644No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC14770.16603714195782185No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC14370.16154053689464457No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC13500.1517604208822339No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT13110.14737623094563604No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12960.14569000404694457No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT12890.14490309816088853No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC12790.14377894689509418No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA12400.13939475695849635No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA11600.13040154683214175No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11580.13017671657898286No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA11560.129951886325824No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA11150.12534286613606727No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11040.12410629974369351No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC11010.1237690543639552No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10970.12331939385763749No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10950.12309456360447862No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10910.1226449030981609No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10860.12208282746526372No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT10630.11949727955393677No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG10550.11859795854130131No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA10200.11466342911102118No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA10110.11365169297180629No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT9990.11230271145285309No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA9960.1119654660731148No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA9930.1116282206933765No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC9920.11151580556679706No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC9780.10994199379468501No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA9770.10982957866810558No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG9710.10915508790862898No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT9680.1088178425288907No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA9590.10780610638967579No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA9570.10758127613651693No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG9530.10713161563019921No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT9500.1067943702504609No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC9450.10623229461756376No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA9220.10364674670623679No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACAC9220.10364674670623679No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG9210.10353433157965736No Hit
CTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACC8950.10061153828859212No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAAC100.007087727145.02054145
ATGCGCC100.0071140034144.841174
AGCGAGC404.809408E-9108.76541145
AGTAGAA13050.0106.2038651
GCAGGTA5050.0103.404272
CCGCACG301.598236E-596.68036145
CAGGTAC5600.091.85513
GGTACTG5700.090.2030265
GTGTCGC259.381116E-487.01234145
CCGCACA502.266097E-887.01234145
GAGCGAA353.422201E-582.929571
CCCTTAC4700.080.282881
GTAGAAA17800.077.8234942
AGCGAAA1350.075.250911
AGGTACT7500.073.386194
GGTCAAA5500.072.563381
TAGAAAC20700.067.1991653
GACAGTA7800.066.8422557
CTTACAC5650.065.396533
CCTTACA6000.062.8563882
Produced by FastQC (version 0.11.5)