FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853946
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG10640.12459804249917442No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT9790.11464425151004864No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA8730.1022312886294918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1850.047.074511
CGAAAGC3000.031.3931243
TATACAG8100.031.2945215
CAGCTCG7550.028.7829889
TATATAC16450.028.625953
GCGTTAT1100.00962334826.3902531
ATACAGC9700.026.1325386
CTATACT2951.4413672E-824.553544
GTATTAG3352.0845619E-923.8300061
ATATACT9750.023.7728634
GTATATA12650.023.521751
TATACGC1550.001808761523.386012
GTATTAA6050.022.7915841
GTTACGC2552.7239894E-622.7280543
ATACTTA10000.022.4515826
TATACTG4058.1490725E-1021.45915
TGTTACG2755.211281E-621.089932
TAGTACC3457.5748176E-820.9950564
TATACTT10400.020.891675
CATATAT10100.020.8378491
Produced by FastQC (version 0.11.5)