FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_B08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_B08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871060
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG10770.12364245861364316No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT9930.11399903565770442No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA8830.10137074369159416No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCG259.41117E-486.9421547
AGCGAAA2650.041.0741271
CGAAAGC3250.033.443133
TATACAG7400.032.3095865
TATATAC17600.026.7608383
GCGAAAG4100.026.528082
GTATATA13450.026.4360641
TAGGACG1701.0358138E-425.5712225
TATACTT11200.024.581865
CAGCTCG7800.024.1505999
CATATAT11200.023.9721341
ATATACT10350.023.8060594
TATAGTA4002.7284841E-1123.5657772
TACCGAG1550.001815312523.3715487
TTATACT4403.6379788E-1223.0581424
ATACTTA9450.023.0005686
CATACAT9000.022.540568
GTATTAG2903.2570824E-722.5199531
GTATAAT3554.081812E-922.484711
GTACTAT3604.796675E-922.1724241
Produced by FastQC (version 0.11.5)