FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATTACCCTAAGGTATATAACACGTCTTTCGATAAGGGCGAAAGGTT13.4482758620689653No Hit
AATTTCAAATAAGCTGAAAAATTAACTCATTAGCTAATCACTTAAAATAA13.4482758620689653No Hit
AGCCAACACGGATATAGTCACTATAAATATACAAACAATATTTTCCAAAA13.4482758620689653No Hit
ATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTCTTCAACAAACAAG13.4482758620689653No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT13.4482758620689653No Hit
GCTAGCCCCAGCTTCCAAGCCATGAGGTTATTGGGATTTATGCGTTTCTC13.4482758620689653No Hit
CTCTTATTCAGTCTATGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT13.4482758620689653No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13.4482758620689653No Hit
CCATTATACTGGAGCTACTCCATACAGCCATGGAACAGGAACAGGATACA13.4482758620689653No Hit
ATGTTCGTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAATTG13.4482758620689653No Hit
TAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACT13.4482758620689653No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAC13.4482758620689653No Hit
TCCCCAGCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGAGCCGAAC13.4482758620689653No Hit
TCTGTATTCAACAGTCTATATGCGTCTCCACAACTTGATGGGTTTTCGGC13.4482758620689653No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC13.4482758620689653No Hit
AGGCAAGTGGAAGATGGCAATGAGATACCCAATTACACATCTGGGGTGGA13.4482758620689653No Hit
AGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGAAT13.4482758620689653No Hit
TCATTAATCTCTGGAATCATGTCCAATATACTCATGTATGCTGTAATTGG13.4482758620689653No Hit
AATTGAAGGAAGAGACCGCACCCAGGGGAGTGGAGGAAGGCTCTATTGGG13.4482758620689653No Hit
GTATGCTAGAGTCCCGGTTTCGTGTGATTATGCACAATACGGCCCCTGGC13.4482758620689653No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT13.4482758620689653No Hit
TATCGCAAGCATGACGACGAACTATTTAACAGCAGAAGTGTCCCACTCCA13.4482758620689653No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATC13.4482758620689653No Hit
GTTTTGATTCTTGCCCTGCTCTCTTCGTCAAGGGTGTAGTCCGCTTTGGT13.4482758620689653No Hit
AAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAT13.4482758620689653No Hit
AAACAGGACAGCAGACTACACCCCAGATGTTCCTTAATCTTAATCTTCAG13.4482758620689653No Hit
GTGAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTATTTTGTTGGC13.4482758620689653No Hit
CGTGGAATGGATCTTGAAAGAGGACTCCAGCGAGACACTTAGAATGACAA13.4482758620689653No Hit
ACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)