FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT11.4084507042253522No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT11.4084507042253522No Hit
ATAAAAGGAAGATACACATCGCTTTTTATGGTTTAACTCCAGCCACTTGC11.4084507042253522No Hit
GGGTGGGGGCGTAGGGGTGGGGGGCCGGGGGCGGGGGCGGGGGGGGGGGC11.4084507042253522No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC11.4084507042253522No Hit
CCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCAAT11.4084507042253522No Hit
AAACAAGGCATTTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC11.4084507042253522No Hit
AGCCCAAATTTCTTCCGGATTTGTATGATTACAAAGAGACCCGGTTCATT11.4084507042253522No Hit
GGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTT11.4084507042253522No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG11.4084507042253522No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATAGAAAAGGGAAAG11.4084507042253522No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTTCCCACACTACGTCCCCGGGCCC11.4084507042253522No Hit
GGTGGAACAGATCTTCATGATCTCAGAGCACTCTTCTTTCTTGATCCGTC11.4084507042253522No Hit
ATATGAACTGCTAGGGAACAATGTCTCGAATAGATTGCAGCACGTCTGGT11.4084507042253522No Hit
TTTTGGACAATATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGT11.4084507042253522No Hit
GCTTTTGTTATTTCTGGGAAAAACAAAAAAGAGACTAGGCACTTAACGAA11.4084507042253522No Hit
GGCTGGCTGTAAGGAAGTATGCTAGAGTCCCGGGTTCGGGTCCTGAAAAA11.4084507042253522No Hit
GGCCAGCTTTGGGTGGACCGGGGCTTCCAGTGTGATCCCTGGTGGGGGTG11.4084507042253522No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA11.4084507042253522No Hit
AATTGATGGGGAGCGGGGGTCGGGGGGTCGCGGGCTGGCGGTCAGTTTGG11.4084507042253522No Hit
ATTTAAAGATGAGTCTTCTACCCGAGGTAGAACCGTCCGTTCTTTCTATC11.4084507042253522No Hit
AGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCT11.4084507042253522No Hit
ATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTC11.4084507042253522No Hit
ACACCAATAATTACGGGCCTGGGTGAGGAATGATTGGCCCAACGATGCAA11.4084507042253522No Hit
GTATAAAAACCAGAGAAAAAAGTATCGAAATGAATCCCAAACCCAATTAC11.4084507042253522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.4084507042253522No Hit
GCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTT11.4084507042253522No Hit
TACCAAGCCACAGCAGGGCTCCGCAGGCCAGGTCAGTGGGCCGCCGGCCT11.4084507042253522No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA11.4084507042253522No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA11.4084507042253522No Hit
CTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAG11.4084507042253522No Hit
CTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC11.4084507042253522No Hit
ATGGAAATGAGGCGCTTCCTTCTTCAGTCTCGTAAGCAGATTGAGAGCAT11.4084507042253522No Hit
GTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAG11.4084507042253522No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC11.4084507042253522No Hit
CCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAG11.4084507042253522No Hit
TAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCA11.4084507042253522No Hit
GCTTCTACAAGTATTGATGCCCCCCGTGAGGCGAGGCACTGGACATCCGA11.4084507042253522No Hit
CATATAGACTGTTGAATACAGATTTTCCCAGTAAGGTCCTGCACACTTTC11.4084507042253522No Hit
GAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCCAATC11.4084507042253522No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCATTGGATGATGATGTGCAT11.4084507042253522No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11.4084507042253522No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT11.4084507042253522No Hit
CTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCC11.4084507042253522No Hit
TCTTCTTGGTCATGTTGTCTCTTACTCTCCGTTTTCTTTGAAAGTGGGTT11.4084507042253522No Hit
TTTTTGTACAGTATTGATAGCAAATAGTAGAATTGCCACAACTACTTCAG11.4084507042253522No Hit
GGCTACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG11.4084507042253522No Hit
TCGGGATTCTGCCCGACGTGGCCCGCAGCACGGAGATGTCGCTGTGCGGG11.4084507042253522No Hit
AGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGACCCTTCTTCTT11.4084507042253522No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG11.4084507042253522No Hit
GTTGGAGCTGACGGGCGGCAATCGAGGCGAGTTATTTTTCGGGCGCAGTT11.4084507042253522No Hit
GACCGCCCCAATACTCAGAAAAGGGAAAGTGGACGACGACCCCAGAGCCT11.4084507042253522No Hit
GAATTTTAGGATTTGTGTTAACGCTCACCGTGCCCAGTGAGCGAGGACTC11.4084507042253522No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11.4084507042253522No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA11.4084507042253522No Hit
GGTAAAAAGAAGGGCTATCGCAAAACCGGGTCTGCAGAGTACAGGTTTCG11.4084507042253522No Hit
CATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAATAGGAGAA11.4084507042253522No Hit
CTAACTACATTACAGTTCCAATGTTAGAAGTTCAATGTGCTCAATTCCTT11.4084507042253522No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGACCG11.4084507042253522No Hit
CTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCA11.4084507042253522No Hit
CTTTCCCAAGTATTTCCATTTTTTGTTAGAACATTATAGCTGCCATGCTA11.4084507042253522No Hit
ATGCTGCAATATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGAC11.4084507042253522No Hit
ATTATTTATGGATAATTACAGCCGCTACGTAGAACATGGAATCACTAAGT11.4084507042253522No Hit
CCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGG11.4084507042253522No Hit
CAACTACACATTCCTGGATTCACAAGAGGAATCGTTCTATTCTCAACCTA11.4084507042253522No Hit
GGGTGGGGGGGGGTGGGGTGGGTGGCGGGGTCGGGGGGGGTGGGTGTGGG11.4084507042253522No Hit
CGATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTG11.4084507042253522No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA11.4084507042253522No Hit
GTACTGACCAATGTCTACCAGTACTGTACACACGAAAAGACAACGTCAAA11.4084507042253522No Hit
CAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCAC11.4084507042253522No Hit
GCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGG11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTT50.0145.03
GTTCTGC50.0145.07
TTCTGCT50.0145.08
TAGTTCT50.0145.05
TCTGCTA50.0145.09
TAGTAGT50.0145.02
CTAGTAG50.0145.01
AGTTCTG50.0145.06
GTAGTTC50.0145.04
AAGCATA50.0145.0145
Produced by FastQC (version 0.11.5)