FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCAGCAGCCACATTAGCATCTCTCTTTGAAATGTGCCCAAGCACCCA14.0No Hit
CTCTTATCCTTCTCAGCGACATCTCCGTGCTTGGGGTCATGTAGGGCAGT14.0No Hit
CTCCCACATCATTGATGACGAATAAGTTATTGTCAACTTCTCAGTTCCTT14.0No Hit
TCATGGGATTACAAAGTCTTCCCCGATTATCATCATCCAGTAGCTCGCAG14.0No Hit
GCGGATGCATTTTGTTGCTTGGGATTTCCTCCACTCCTGGTCCTTATGGC14.0No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG14.0No Hit
ATCCTGACCACTCATTTATTCCTACGAGATCTTGCTTTATTGAGAAGTTA14.0No Hit
GAGATCTGCATGGCCAGGGTTTGTATCAACTCTCCTCCTTATTTTAACTT14.0No Hit
TAGATTATTACCACCCTACATCGACGCCATGTTGGATCATACAGAATTGG14.0No Hit
TTCTCACATATAGGAACATGGGCCTCGCCACTTGGCCTATCGCAGTCCTC14.0No Hit
GATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTC14.0No Hit
GTTAAAAAGAAGGGCTATCGCAACCCCTGGGATGCAGATTATAGGTTTCG14.0No Hit
ACTCCAAACTTAAAAAGTACAACACATCACAATCAGTACTCAAATGAGAG14.0No Hit
GCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACATGAGGGGTATG14.0No Hit
ACTTTGAAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTG14.0No Hit
ACACGAAGTGGAATGAAAATAAAACTCCTCGAATCTTCCTGGCGATGATT14.0No Hit
CATTAGCCTTCTCTCCTTTTGAAAGATTGCTCAGTTCATTTATGCTTAAT14.0No Hit
GGCGTGCGGTCGGGGGGGGGGGGGACGGTGGGGGGGTTGGGTCCGCGGGG14.0No Hit
GGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGAATGCA14.0No Hit
GCTGGGTAGTACATGCGGGGTGGTTGAGGCGGTCGGGGGGTGTTGGGTTG14.0No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT14.0No Hit
CTATAAATAAGAATGTTAAATTTGAATTCAAAATATGTTTTGATCTATAT14.0No Hit
TAACCGTACCATCCATGTACCATCCCTGTCCACCCCCCTGCAAGGAAACC14.0No Hit
TCACAACACAAAGAGATTTCTTTCACAATCACACGCAACCACACTAAGTT14.0No Hit
GGGCAAACACTCGAATGCACACAATGCAACAACTATATAGTAATTAAAAA14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)