FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG60.5905511811023622No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA50.4921259842519685No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.39370078740157477No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA40.39370078740157477No Hit
ATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACTAAT40.39370078740157477No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.39370078740157477No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.2952755905511811No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.2952755905511811No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG30.2952755905511811No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT30.2952755905511811No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT30.2952755905511811No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT30.2952755905511811No Hit
ACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATT30.2952755905511811No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC30.2952755905511811No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC30.2952755905511811No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA30.2952755905511811No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC30.2952755905511811No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT20.19685039370078738No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.19685039370078738No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG20.19685039370078738No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTG20.19685039370078738No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA20.19685039370078738No Hit
TTACGGACCAATTCTCTTTCTAGCATGTACGCCACCATCAAGGGAGCAAT20.19685039370078738No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG20.19685039370078738No Hit
TATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGA20.19685039370078738No Hit
GACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTG20.19685039370078738No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGAC20.19685039370078738No Hit
CGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCA20.19685039370078738No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.19685039370078738No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA20.19685039370078738No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT20.19685039370078738No Hit
ATGTGATGGGAATGATCGGAATACTGCCCGACATAACCCCAAGCACGGAG20.19685039370078738No Hit
CTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACT20.19685039370078738No Hit
GGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAG20.19685039370078738No Hit
GAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGT20.19685039370078738No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG20.19685039370078738No Hit
TTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGC20.19685039370078738No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20.19685039370078738No Hit
GCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCG20.19685039370078738No Hit
AGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGA20.19685039370078738No Hit
GAGTAGATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGA20.19685039370078738No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC20.19685039370078738No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA20.19685039370078738No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20.19685039370078738No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA20.19685039370078738No Hit
ACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTT20.19685039370078738No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA20.19685039370078738No Hit
CAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCA20.19685039370078738No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAAT20.19685039370078738No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA20.19685039370078738No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC20.19685039370078738No Hit
GCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGG20.19685039370078738No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT20.19685039370078738No Hit
GATGGATGCTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGG20.19685039370078738No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.19685039370078738No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT20.19685039370078738No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.19685039370078738No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC20.19685039370078738No Hit
GATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAA20.19685039370078738No Hit
TGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAA20.19685039370078738No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG20.19685039370078738No Hit
GGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGC20.19685039370078738No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.19685039370078738No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT20.19685039370078738No Hit
ATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAAACAAG20.19685039370078738No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20.19685039370078738No Hit
CTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTC20.19685039370078738No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA20.19685039370078738No Hit
TCTCTAGGTAATATATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATG20.19685039370078738No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.19685039370078738No Hit
CTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCTCT20.19685039370078738No Hit
GTCCAGCCAAAAGCATGGAATATGATGCCGTTGCAACTACACATTCCTGG20.19685039370078738No Hit
GTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGC20.19685039370078738No Hit
GTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTC20.19685039370078738No Hit
GTAATATATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATGAACCGGT20.19685039370078738No Hit
GAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGTGCATGG20.19685039370078738No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA20.19685039370078738No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20.19685039370078738No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG20.19685039370078738No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA20.19685039370078738No Hit
TCTCAAATGAGACCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCC20.19685039370078738No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.19685039370078738No Hit
GGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAAC20.19685039370078738No Hit
TCCCACAGCTTCTTTAACTCAAATGATCTTCTCGTCTGAATTTGTGTGTC20.19685039370078738No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA20.19685039370078738No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC20.19685039370078738No Hit
ATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGA20.19685039370078738No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT20.19685039370078738No Hit
ATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG20.19685039370078738No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC20.19685039370078738No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20.19685039370078738No Hit
ATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATCAAACCCA20.19685039370078738No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG20.19685039370078738No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)