FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717165
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG31490.439090028096742No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA26360.3675583722016551No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20870.2910069509805972No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20430.28487168224885484No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19720.27497158952263423No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC19560.2727405827110916No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT18980.2646531830192494No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT18820.2624221762077067No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG16620.23174583254899503No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG16350.22798100855451675No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT14590.20343993362754736No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG14520.20246386814749745No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG13640.19019333068401276No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC13450.1875440100953058No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13140.1832214343979419No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT13060.18210593099217057No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG12920.18015380003207074No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC12720.1773650415176424No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG12370.17248471411739277No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT12060.16816213842002886No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10760.15003520807624465No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10520.14668869785893066No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10450.14571263237888074No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG10110.14097174290435255No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT9460.13190827773246044No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA9190.1281434537379822No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC9120.12716738825793228No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG9110.12702795033221087No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA8550.11921942649181151No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA8440.11768560930887591No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC8410.11726729553171167No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC8230.11475741286872618No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC8180.11406022324011908No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA8170.11392078531439766No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA8160.11378134738867623No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG8010.111689778502855No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA7960.1109925888742479No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT7780.10848270621126241No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG7770.10834326828554099No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG7710.10750664073121248No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT7430.10360237881101281No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA7230.10081362029658446No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG7180.10011643066797737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG100.0070880065145.0167145
GACACGC100.0070880065145.0167145
AGCGAAA1150.0107.223661
CAGGTAC3150.0103.474993
AGTAGAA6050.0101.90681
GCAGGTA3600.092.6042942
GGTACTG4050.080.4637155
AGGTACT4650.076.316084
CCGCACG502.3039956E-672.50835145
GCGAAAG1750.070.4022752
GTAGAAA9500.064.84422
CGAAAGC1900.064.808033
GACAGTA4100.063.5903937
TTTAAAC9800.055.4330373
AGCGAGC400.006047434654.381256145
GGTCAAA6250.053.3847771
TTTTAAA10650.050.3567772
CAAGGTA2400.048.2849669
TAGAAAC13100.048.1040233
AAATATA6950.047.9309045
Produced by FastQC (version 0.11.5)