FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011523
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11630.11497514144512778No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10690.10568222373589131No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10120.10004715661433303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2900.040.040931
GTACCGT1851.6546255E-731.3275786
CAGCTCG7000.031.0463319
CTATACG1204.0773363E-430.1839319
TATACAG9200.028.3480515
CAATTCG2054.0678788E-728.2698299
CGAAAGC4200.027.6035633
GCGAAAG4350.026.668862
GTATATA16550.025.8723721
TAGTACG1751.262224E-424.8382934
CATACAT10600.023.9193448
TATACAT11950.023.6431454
TACATAC10850.023.3693646
TATATAT10250.022.636012
TATATAC20600.022.5116443
TACAGCT11150.021.4411287
GTATAGA7450.021.4313071
ACAGCTC13850.020.3986728
GGTCGTT2155.0821144E-420.2172167
ATACAGC12900.020.2172166
Produced by FastQC (version 0.11.5)