FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATCATTGATGACTAATAAGTTATTGTCAACTTCTCAGTTCCTTGC13.225806451612903No Hit
GGAGGTGCTCTTTGATGCTGGGTTCAACTCTCCCTAGGAGATCTGGAGCT13.225806451612903No Hit
TTGTTGAACAACTTCCATTGTTTCAAGGCATGAATTCTCAAATATTCCTG13.225806451612903No Hit
ATCCAGAACAGCTTAACAATGGAGAGAATGGTGCTCTCTGCTTTTGACGA13.225806451612903No Hit
AATACGGACTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGAT13.225806451612903No Hit
CGCTCAACGAGCCCAGTGAGCCAGGACTGCAGCGTAGACGCTTTGTCCAA13.225806451612903No Hit
AGCTTACAAATATGTGAGCTAAAATTACTTTGTTGTAATCCAAGATCTCC13.225806451612903No Hit
CAAAGGGCCTCTGCGGGCCAAATCAGCATACAACATACGTTTTCTGTGCA13.225806451612903No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG13.225806451612903No Hit
TCCTTAGACAGCATCGACCTGAGGACCAACCCGTAGACATATGAAAGGCA13.225806451612903No Hit
TCTCAAATGAATCTGGGAGGCTAATAGACTTCCTTAAAGATGTAATGGAG13.225806451612903No Hit
GCATTTGATTGCAACATATAGTTGTAATCCCTAACCCTCAAGACTAGGCT13.225806451612903No Hit
CTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTATTCTCGCAACCATTGT13.225806451612903No Hit
CATACTACAAATATCACGAAAATAGTCACGCAAATCAAACGAATTAAAAT13.225806451612903No Hit
GTATAGAGCAGATAAACAAAACAAGCAGCTCAAAAGAGAAAGAACGGTCC13.225806451612903No Hit
GGAAGGGCCTCTAGCCATGTGCATCCACGCGCCGTGGATCGGAAACAGGA13.225806451612903No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC13.225806451612903No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC13.225806451612903No Hit
GTAGAATGTTTGTTGTACAGCTTGTATTACCTCAAGGGAGTGAGAGATCA13.225806451612903No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG13.225806451612903No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC13.225806451612903No Hit
GGCTTCTTCAGGAACCTAAGTGGGCGGTTCGCAGTGTGGAGACTGTATAA13.225806451612903No Hit
AACTAATCCCCTTAATCACATTTTCAGTAATAAAAATAATCATTCTATAA13.225806451612903No Hit
GAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAG13.225806451612903No Hit
ATATAGTGGAATTCCCTCACCCTCGTGACTCGGGTCTTCAATAAATAATT13.225806451612903No Hit
GATTTGAAATCACAGGAACAATGCGAAGGCTCGCTGCCCAAAGCCTCCCG13.225806451612903No Hit
CTCTCAATATAAGCACAACGTAAATAATCTCAGTCAACATATTATATAGC13.225806451612903No Hit
AGTGTTTCCATAATTGCCTGACCAATGATAATAAATTTTCTAGCTTCAGC13.225806451612903No Hit
ATCCCGACCAGTGAGTACACTTACCTTTCACAGTCATGCCCACTTTATAC13.225806451612903No Hit
TAACAATTAGAAGCAATTTTCTAGATTAAGCAGAAAACCACGCCAGTTGT13.225806451612903No Hit
GTAAGTCCAAATGTCCAGGAAACCATCATCAACTTTTTTATTTAAATTCT13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)