FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAACTCTCCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAACCA13.571428571428571No Hit
GACGACTCCTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGACGATG13.571428571428571No Hit
ATACAACTTACAAGACGACAAGAGCTCTTGTCCGCACCTGAATGGATCCC13.571428571428571No Hit
GGATTGAGAATAGCCAAATATATTCTCAGAAAGGCAACCAGTAGATTGAT13.571428571428571No Hit
CCATTTTATTGTATAGCAGTGTAGGTTTCTGAGACCACTGAATTTTAACA13.571428571428571No Hit
CAGTGATGCTCACTGCTGGACCATCTCTCCTATATTCTTTGCCCAGAATG13.571428571428571No Hit
ATGTAGCTCCCATTGAACACATTGCACGCATGAGAAGGCATTACTTCACA13.571428571428571No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGAAGCACTTAT13.571428571428571No Hit
ATCAAGTACAATTGAAATCAGCAGCACCTACTGGGCAATAATCACCAGAA13.571428571428571No Hit
TTGTTTCTACTAATAACCCGAGAAGAAACAAGCTCGATTTTAAACAATTC13.571428571428571No Hit
TTTTTGGACAGTATTGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13.571428571428571No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13.571428571428571No Hit
GAGTAAAGGGGTTTTCATACAGGTATGGTAATGGTGTTTGGATAGGAAGG13.571428571428571No Hit
CATTACTATAGAATTCACTCTAAACAAAAACAAAAAGATAAACAATGAAA13.571428571428571No Hit
GCATTCCCAGTGAATCCTGCCATGATGGATCTTTTGTCAAATCGGAGCTT13.571428571428571No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC13.571428571428571No Hit
TCCAAATTCACCCATAGAGCTTAGACATTATACACCGTTCCAGTAATAAC13.571428571428571No Hit
GGGGTGAATCTCCTTTTATAATGTGAAGACACTACATAGACATAACCATT13.571428571428571No Hit
CATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGA13.571428571428571No Hit
ACCTCATACAAACGCGAACTCATTCCGAATAATTCCCTCGATTCAGTCCG13.571428571428571No Hit
CTCAAGAGTTGAGGCAGTCGCTTGGGCAGCTAGGGCTTGTCATGCTGGCA13.571428571428571No Hit
GCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTACCCCTGCAGTG13.571428571428571No Hit
GAGTTGAAACATGCATCAAAGAGCCCATCCTGGGATAAATTCCGGTGCCT13.571428571428571No Hit
GTTATCATCATCAGAATGAACAAGGATATGGCTATGCTGCGGATCAAAAA13.571428571428571No Hit
CATCTGTACCATAGCATTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTT13.571428571428571No Hit
GATCTATGCAGGGAGTCAGCAATCTGGTCACATGTTGCGCATATTAGCCC13.571428571428571No Hit
TTTTAGAGCCTATCTGCATGGATTCGAACCGCACGGCTACATTCAGGGCA13.571428571428571No Hit
ATGCCGCCTTTGAAATTTAAGCTGCAAGATGGATATGCAGAGTGCACAAT13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)