FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA30.2555366269165247No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.2555366269165247No Hit
GTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGG20.17035775127768313No Hit
GGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAA20.17035775127768313No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGAATGCCCCATAGCACGAG20.17035775127768313No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20.17035775127768313No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.17035775127768313No Hit
TAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATT20.17035775127768313No Hit
GAAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACG20.17035775127768313No Hit
CTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACAT20.17035775127768313No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.17035775127768313No Hit
TCTCTAACCCTTAAAAATCGGTCAATACTCACTACCACTCTCTCCGTGCT20.17035775127768313No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.17035775127768313No Hit
GCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGCAAGGCCGTATTT20.17035775127768313No Hit
GAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCC20.17035775127768313No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA20.17035775127768313No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT20.17035775127768313No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.17035775127768313No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA20.17035775127768313No Hit
AAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTC20.17035775127768313No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.17035775127768313No Hit
CTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTCCTGTA20.17035775127768313No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.17035775127768313No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG20.17035775127768313No Hit
ACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGAC20.17035775127768313No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.17035775127768313No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA20.17035775127768313No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA20.17035775127768313No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA20.17035775127768313No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC20.17035775127768313No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)