FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTCGGCAAGCTTGCGCATAGTTCCTGTAATCTCAAATTTTTCTTCAA14.0No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT14.0No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT14.0No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC14.0No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT14.0No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG14.0No Hit
TATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGA14.0No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC14.0No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA14.0No Hit
CTATACAAGAAGATAAAAAGAGAAATAACGTTAAATGGGGACAATGATTG14.0No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT14.0No Hit
ATAAAATCTTACACATCAGTAATGCAATATAAGAACCCCGCACTCAGAAT14.0No Hit
TACTTTGTAATACAATTACTAACGTTTTAATGCAGAAAGAGATTTATTAT14.0No Hit
CTTGTGAGCTGGGTTTTCATTTGGTCTCATCAGGCTGACCACTTGGCTGT14.0No Hit
CCAATAATCAGGCAGTACAAAAGACTTTTGCGGTCTAGAACTACTGCAAA14.0No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC14.0No Hit
ATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCC14.0No Hit
ACCATGGGCTGGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTAT14.0No Hit
GAATTGCGCTCACTCAGCGCCATTTGTCTACGGCATCATTTAACCAGTTA14.0No Hit
CCCATAGACTCCTACTGGCCATTTCTTGTCTTATATTGAAAAGCCTAGTT14.0No Hit
ACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAA14.0No Hit
GTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATT14.0No Hit
ATATATTGATATACGAACAACACGCAGCTAAGGACTACTACATAGAAATT14.0No Hit
GCCCAGATCTCTAGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTG14.0No Hit
GGATGGTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGAC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)