FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_H07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_H07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894353
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAATACACCAGTGTGTTTACCAATAATGAGGACACAGGGACTTTTT9420.10532753845517374No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA100.0070845024145.042662
AGCGAAA3700.0109.817351
CTAACCG300.001941589672.444264
CCGATCA1050.068.982968
GCCGATC1150.062.9809237
AGCCGAT1150.062.97746
GCGAAAG6750.061.2402342
CGAAAGC7500.055.0822873
GAGCGAA901.3161534E-648.3719331
CGGAGAC604.9389916E-448.344845145
CGTACCT450.00967454348.2880745
GGACCGT1307.414201E-944.5711147
AGGACCG1553.5019184E-837.3801356
GATACGC1001.384812E-436.278951
AAGCGAC800.00203411936.258636145
CGGTGTT850.002738628834.12578145
CCTAGAC3650.031.7706033
ATGTGCG1255.18344E-428.9712227
CTAATGG2903.9108272E-1027.5219611
GTGCGTG1958.8170145E-626.001279
Produced by FastQC (version 0.11.5)