FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_H04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_H04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831968
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG28090.3376331781991615No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA26030.31287261048501863No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG23750.2854677102965499No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC20580.2473652832801262No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC18440.2216431401207739No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA16740.20120966191007347No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA15850.19051213508211853No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT15590.18738701488518789No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC15310.18402150082695487No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA14810.17801165429439594No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC13740.1651505827147198No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC12500.1502461633139736No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG12400.14904419400746183No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC11830.14219296896034464No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT10530.12656736797569137No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA10460.12572598946113311No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC10340.12428362629331897No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA10110.12151909688834187No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA9700.11659102273164353No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG9280.111542751644294No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT9160.11010038847647986No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT9030.10853782837801454No Hit
CTTCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCG9010.10829743451671218No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA8720.104811723527828No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG8710.10469152659717681No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA8430.1013260125389438No Hit
CTTGTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGAT8400.10096542174699027No Hit
CTCCAGAAGTTTCTGTCATTGATTCCACGTTTGATCATCCTGATCAACTC8360.10048463402438554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC100.0070889336145.01173145
CCGACGC100.0070889336145.01173145
CCGTATA203.864581E-4108.7588145
AGCGAAA700.0103.6359251
GTAGAAA4850.071.7972641
GGGTAGA1350.064.484581
AGGGTGT1950.063.141866
GGCAGCC502.0092417E-458.004692145
TAGATCC502.0092417E-458.004692145
AAGGGTG2200.055.966655
CAAGGGT5050.055.9340554
TTAGTAG651.0949083E-555.756972
CTGTCTA400.00604859254.3794145
TCGGTCC400.00604859254.3794145
GGTCGTT805.9132435E-754.320577
ACAAGGG5400.052.3118443
AAGTGTT858.9848436E-751.1252447
CGTACTC6750.047.214793
GTACTGA1255.2405085E-946.353556
TAGATAT1101.0030635E-746.0929533
Produced by FastQC (version 0.11.5)