FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_H03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_H03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082335
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT26420.24410187234081868No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA23530.21740034277742104No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG22590.20871541620662734No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC18990.17545399529720465No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT18830.17397570992345254No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG18600.17185067469868387No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18570.17157349619110535No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA18050.16676906872641095No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT17730.1638124979789067No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG17610.16270378394859264No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC17370.16048635588796445No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC15660.14468718095598868No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC15270.14108386035746787No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT14480.13378482632456679No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC14290.13202936244323615No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA13980.12916518453159143No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG13910.12851843468057486No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA13880.12824125617299634No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT13840.12787168482955832No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA13270.12260529318556639No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC12990.12001829378150018No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA12740.11770847288501249No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC12580.11623018751126038No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC12140.11216490273344205No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG11670.1078224394480452No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCA11530.10652893974601209No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG11280.1042191188495244No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG10870.10043101257928459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1400.098.433551
CAGCGCG451.2319579E-680.566505145
GGGTAGA1650.070.332111
GTAGAAA7600.067.758281
GTGGTCC451.1930994E-464.4532145
TAGATAT2950.061.3837173
CAAGGGT5000.050.6982654
AAGGTAC1457.2759576E-1249.9536489
GATAGCA5550.049.586756
TTAGTAG752.544495E-548.353321
AAGGGTG2900.047.4471975
ACATAAT2350.046.2657622
CCATAGG1751.8189894E-1245.590281
GTTTAAA803.7257454E-545.331241
TAATGTA4900.044.3384135
GGATAGC6300.043.681545
ATATTCA2500.043.4556544
TGTTATG1351.0362783E-842.9152035
CAAGGTA4300.042.110148
GTATTAA5200.041.8442231
Produced by FastQC (version 0.11.5)