FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029445
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT11090.10772795049759823No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA10950.10636799440475209No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA10400.10102530975428507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1800.088.603951
TACGCGA350.003577994962.092552
ACGCGAT350.003582818762.071433
CGCGATC400.006078527354.3125044
GTACGCG553.2203295E-452.7230071
GGTCGTT1402.746674E-1046.5558367
TCGTACT3000.045.8794942
CTAGTAC2200.039.5000043
CGTACTC3750.034.7600023
GTCGTTT1905.486072E-934.3043028
GTATTAA5750.032.779961
GTACTGA3100.032.7027136
GTAGAAA7300.031.7782541
CGATCAT950.004755154330.4897526
TAGTACT3900.029.7094044
TATAGAC2951.4551915E-1129.4676482
TATAGTG5550.028.7154712
AGTGGTA5300.028.6920035
ATATAGT5600.028.4798411
GTATACC4200.027.5886449
Produced by FastQC (version 0.11.5)