FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041652
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA99920.959245506176727No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC79920.7672428027786631No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG74950.7195301309842442No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT73360.7042659160640982No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG62730.6022164792080273No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA60700.5827282048131237No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG59100.5673679885412787No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG57880.5556558236339968No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA55340.5312714803024426No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC45890.4405502029468575No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC42090.40406968930122533No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG41630.39965362712306984No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC40590.38966948654637057No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT39950.3835254000376325No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG38440.3690291959310787No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT35060.33658073905680597No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT33860.3250605768529221No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA33690.32342855387403857No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT33500.32160452819175694No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG32680.31373241735243634No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC32640.3133484119456402No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG32510.3121003943735528No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT31970.3069163213818051No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT27030.2594916536424833No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG26360.25305956307864813No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG26230.2518115455065607No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG25520.24499544953592947No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC25360.24345942790874497No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG24750.237603345455104No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG24000.23040324407767662No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA23750.22800321028520082No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC22970.2205151048526763No Hit
CCCTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA22830.21917108592888987No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC22550.21648304808131702No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA21780.20909094400049155No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20220.19411473313544256No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20110.1930587182667532No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA20020.19219470610146192No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA19200.1843225952621413No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG19190.18422659391044224No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA19110.18345858309685No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA18740.17990653308398583No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA18580.1783705114568013No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT18340.17606647901602454No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC18240.17510646549903422No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA17880.17165041683786908No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA17700.1699223925072865No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG17640.16934638439709232No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG17550.16848237223180104No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG16810.16137827220607265No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG16730.1606102613924804No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC16710.16041825868908235No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT16490.15830622895170363No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA16390.15734621543471333No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT16370.15715421273131527No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT16090.15446617488374237No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG16000.15360216271845106No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA15610.14985811000218882No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT15540.1491861005402956No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG15140.14534604647233434No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC14920.14323401673495564No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC14910.1431380153832566No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA14750.14160199375607208No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC14570.13987396942548952No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG14480.1390099572601982No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA14470.1389139559084992No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC14470.1389139559084992No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG14400.13824194644660598No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC14390.13814594509490694No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT14350.13776193968811082No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA14230.1366099234677224No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG14150.13584191265413018No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA14110.13545790724733403No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA13990.13430589102694568No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT13670.13123384777257663No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA13620.13075384101408147No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA13490.12950582344199407No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT13250.12720179100121728No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT13220.1269137869461202No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT13060.12537776531893569No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA12870.12355373963665409No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC12830.12316973422985796No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC12830.12316973422985796No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA12800.12288173017476085No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCATGATTGAGGC12700.12192171665777055No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12680.12172971395437249No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT12610.12105770449247927No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG12410.11913767745849861No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA12360.11865767070000346No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA12200.11712164907281895No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC12190.11702564772111991No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT12080.11596963285243056No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT12040.11558562744563444No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11980.11500961933544024No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA11810.1133775963565567No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT11810.1133775963565567No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC11760.11289758959806154No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT11680.11212957878446929No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA11500.11040155445388672No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA11470.11011355039878962No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT11320.10867353012330415No Hit
GCCTAAATTTCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATG11260.10809752201310995No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC11020.10579348957233319No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11020.10579348957233319No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT10930.1049294774070419No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA10740.10310545172476028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA15050.0117.5572361
GCAGGTA11450.0114.561722
GAGCGAA458.367351E-11112.792841
CAGGTAC12100.0108.9280243
GGTACTG12850.0102.555575
GTACGCT651.6370905E-1189.242681
TACGCTA651.6370905E-1189.195522
ACGCTAG651.6370905E-1189.131293
AGCGAAA2450.088.787371
CGCTAGA703.092282E-1182.7607964
AGGTACT17000.080.935194
CCGCACG554.3875843E-879.10908145
ACTGATC16800.078.015558
GTAGAAA22650.077.110822
GTACTGA18000.074.42016
GAAGGCG608.019197E-872.51666145
GACAGTA15650.072.177417
TACTGAT18650.071.438127
TAGAAAC25450.069.14683
CCGCACA754.8457878E-967.68221145
Produced by FastQC (version 0.11.5)