FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938963
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT13790.14686414693656724No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG13110.13962211503541672No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10960.11672451417148492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3250.037.9584471
TATACCG1251.2726534E-534.7693865
CGAAAGC3950.033.0159453
TATACAG9350.027.8898855
TATATAC19900.027.6699833
CAGCTCG9450.027.5962249
TATAGAC3204.1836756E-1127.1896482
TATATGT9150.026.9161954
CGTGCTA1358.123993E-426.8282328
TACCTAT4350.026.6432137
ATATATG8500.026.4235483
CATATAT10700.025.771681
GTATATA17450.025.367481
GTATAGG3451.07320375E-1025.2409131
GCGAAAG4900.025.155052
ATACTTA10550.024.0309776
ATATACT10950.023.814654
GTATTAG4002.7284841E-1123.5844761
TATATAT10500.023.4780462
TATACTT10350.022.3957425
Produced by FastQC (version 0.11.5)