FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964755
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT11700.12127431316759177No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11650.12075604687200377No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10450.1083176557778918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3200.045.3303451
CGAAAGC4550.036.6152653
ATATACT9100.031.040164
ATACTTA9100.029.4437796
TATACAG8650.029.30275
TATATAC17300.028.4713863
CAGCTCG8200.028.258359
GCGAAAG5500.027.672612
GTATATA15200.027.1982061
CTATACT3900.025.9994494
TATATGT8700.025.8073374
TACTTAC9150.025.3244257
TATACTT10950.024.4705265
CATATAT11550.023.8622071
CTTACAC10600.023.2264989
TATATAT10700.023.0297182
ACTTACA11050.022.9359368
ATATATA12500.022.6124742
ATATATG8800.022.2242343
TATAATA4900.022.1864932
Produced by FastQC (version 0.11.5)