FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAAAAGTCTA15.555555555555555No Hit
GGGCACCGGTTCGTCGGTGACCCTCTGAACATGCATTGTGTAACTTCTTG15.555555555555555No Hit
ATTTCATCATTATCTTGGAGGTTCCATTGGTTCTCACATAATCGTACATT15.555555555555555No Hit
AGTGTGTGCCACTTGTGCACAGATTGCTGATTCCCAGCATCGGTCTCACA15.555555555555555No Hit
GGCTTGTACCCAAATCTGAGCAAGTCCTATGTGAACAACAAAGAGAAAGA15.555555555555555No Hit
ATGCAATAAGCACCACATTCAATTATAATGTAGATCCTCAATAAATAAAT15.555555555555555No Hit
CAGCTACACGTGTGGTAACCTCCTCCCCACTCAATTGGACACACCAGAGT15.555555555555555No Hit
CCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGANNNNNNNNNN15.555555555555555No Hit
GCGTAATGCGGGCCAATATCTCTTGTATTCTTTTCACAAAATGAGAAATC15.555555555555555No Hit
ATATGGAAGCGTTTATAAACTGTATACTTGATTAAGTTCTAGAAGAGAGG15.555555555555555No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG15.555555555555555No Hit
GTATTAATTAGCCCTGCATTGGGACACTTATGCTGTAAAATAGTTCCTCC15.555555555555555No Hit
CTAAAAAACAAAGCCAAGTAAATTGGAAAAGGATGCTTTGAATTTTACCA15.555555555555555No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT15.555555555555555No Hit
GTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAA15.555555555555555No Hit
GACAAAACTACTGCAGTGCATGTGTGAGGAAGGAGTTGAACAAAGATGCA15.555555555555555No Hit
GCTTCAATCCAAGGATCTTCGAGCTTGCTTAAAAGGCAATGAAAGAATAT15.555555555555555No Hit
GAACAGGAACAGGATACACCATGATGCCGTTGCAACTACACATTCCTGGA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)